Hybrid lipid-biopolymer nanocarrier as a strategy for GBM photodynamic therapy (PDT)

Hellen Franciane Gonçalves Barbosa; Henrique Luis Piva; Flavia Sayuri Matsuo; Sarah Caroline Gomes de Lima; Lucas Eduardo Botelho de Souza; Mariana Kiomy Osako; Antonio Claudio Tedesco

doi:10.1016/j.ijbiomac.2023.124647

Hybrid lipid-biopolymer nanocarrier as a strategy for GBM photodynamic therapy (PDT)

Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124647. doi: 10.1016/j.ijbiomac.2023.124647. Epub 2023 May 3.

Authors

Hellen Franciane Gonçalves Barbosa¹, Henrique Luis Piva¹, Flavia Sayuri Matsuo¹, Sarah Caroline Gomes de Lima², Lucas Eduardo Botelho de Souza², Mariana Kiomy Osako³, Antonio Claudio Tedesco⁴

Affiliations

¹ Department of Chemistry, Center of Nanotechnology and Tissue Engineering, Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil.
² Gene Transfer Laboratory - Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, 14040-901, Brazil.
³ Laboratory of Cell and Tissue Biology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, 14049-900, Brazil.
⁴ Department of Chemistry, Center of Nanotechnology and Tissue Engineering, Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil. Electronic address: atedesco@usp.br.

PMID: 37146851
DOI: 10.1016/j.ijbiomac.2023.124647

Abstract

Glioblastoma (GBM) is the most common brain cancer characterized by aggressive and infiltrated tumors. For this, hybrid biopolymer-lipid nanoparticles coated with biopolymers such as chitosan and lipidic nanocarriers (LN) loaded with a photosensitizer (AlClPc) can be used for GBM photodynamic therapy. The chitosan-coated LN exhibited stable physicochemical characteristics and presented as an excellent lipid nanocarrier with highly efficiently encapsulated photosensitizer chloro-aluminum phthalocyanine (AlClPc). LN(AlClPc)Ct0.1% in the presence of light produced more reactive oxygen species and reduced brain tumor cell viability and proliferation. Confirm the effects of in vivo LN applications with photodynamic therapy confirmed that the total brain tumor area decreased without systemic toxicity in mice. These results suggest a promising strategy for future clinical applications to improve brain cancer treatment.

Keywords: Chitosan; Glioblastoma; Nanoparticle.

MeSH terms

Animals
Brain Neoplasms* / drug therapy
Cell Line, Tumor
Chitosan* / therapeutic use
Glioblastoma* / drug therapy
Lipids
Mice
Nanoparticles* / chemistry
Photochemotherapy* / methods
Photosensitizing Agents / chemistry
Photosensitizing Agents / pharmacology

Substances

Photosensitizing Agents
Chitosan
Lipids