Angiogenesis coupled with osteogenesis in a bone tissue engineering scaffold enhances bone repair in osteoporotic bone defects

Guiyu Feng; Wei Liu; Yao Yu; Bingbing Tian; Yingkai Zhang; Fenghe Yang; Jian Huang; Pingxin Zhang; Wei Wang; Dongyang Li; Song Sun; Xufeng Niu; Limin Chai; Jinyu Li

doi:10.1088/1748-605X/accf55

Angiogenesis coupled with osteogenesis in a bone tissue engineering scaffold enhances bone repair in osteoporotic bone defects

Biomed Mater. 2023 May 5;18(4). doi: 10.1088/1748-605X/accf55.

Authors

Guiyu Feng¹, Wei Liu¹, Yao Yu², Bingbing Tian³, Yingkai Zhang¹, Fenghe Yang², Jian Huang⁴, Pingxin Zhang¹, Wei Wang¹, Dongyang Li¹, Song Sun¹, Xufeng Niu², Limin Chai¹, Jinyu Li⁴

Affiliations

¹ Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, People's Republic of China.
² Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, People's Republic of China.
³ Operating Room, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, People's Republic of China.
⁴ Department of Orthopedic, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, People's Republic of China.

PMID: 37144422
DOI: 10.1088/1748-605X/accf55

Abstract

Increased life expectancy has resulted in an increase in osteoporosis incidence worldwide. The coupling of angiogenesis and osteogenesis is indispensable for bone repair. Although traditional Chinese medicine (TCM) exerts therapeutic effects on osteoporosis, TCM-related scaffolds, which focus on the coupling of angiogenesis and osteogenesis, have not yet been used for the treatment of osteoporotic bone defects.Panax notoginsengsaponin (PNS), the active ingredient ofPanax notoginseng, was added to a poly (_L-lactic acid) (PLLA) matrix. Osteopractic total flavone (OTF), the active ingredient ofRhizoma Drynariae, was encapsulated in nano-hydroxyapatite/collagen (nHAC) and added to the PLLA matrix. Magnesium (Mg) particles were added to the PLLA matrix to overcome the bioinert character of PLLA and neutralize the acidic byproducts generated by PLLA. In this OTF-PNS/nHAC/Mg/PLLA scaffold, PNS was released faster than OTF. The control group had an empty bone tunnel; scaffolds containing OTF:PNS = 100:0, 50:50, and 0:100 were used as the treatment groups. Scaffold groups promoted new vessel and bone formation, increased the osteoid tissue, and suppressed the osteoclast activity around osteoporotic bone defects. Scaffold groups upregulated the expression levels of angiogenic and osteogenic proteins. Among these scaffolds, the OTF-PNS (50:50) scaffold exhibited a better capacity for osteogenesis than the OTF-PNS (100:0 and 0:100) scaffolds. Activation of the bone morphogenic protein (BMP)-2/BMP receptor (BMPR)-1A/runt-related transcription factor (RUNX)-2signaling pathway may be a possible mechanism for the promotion of osteogenesis. Our study demonstrated that the OTF-PNS/nHAC/Mg/PLLA scaffold could promote osteogenesis via the coupling of angiogenesis and osteogenesis in osteoporotic rats with bone defects, and activating theBMP-2/BMPR1A/RUNX2signaling pathway may be an osteogenesis-related mechanism. However, further experiments are necessary to facilitate its practical application in the treatment of osteoporotic bone defects.

Keywords: angiogenesis; magnesium; nano-hydroxyapatite/collagen; osteogenesis; osteoporosis; poly (L-lactic acid).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone and Bones / metabolism
Osteogenesis*
Osteoporosis* / metabolism
Osteoporosis* / therapy
Polyesters / pharmacology
Rats
Tissue Engineering / methods
Tissue Scaffolds

Substances

Polyesters