A comprehensive review and update on human fertility cryopreservation methods and tools

Sevastiani Antonouli; Valentina Di Nisio; Christina Messini; Alexandros Daponte; Singh Rajender; George Anifandis

doi:10.3389/fvets.2023.1151254

A comprehensive review and update on human fertility cryopreservation methods and tools

Front Vet Sci. 2023 Apr 18:10:1151254. doi: 10.3389/fvets.2023.1151254. eCollection 2023.

Authors

Sevastiani Antonouli¹, Valentina Di Nisio², Christina Messini³, Alexandros Daponte³, Singh Rajender⁴, George Anifandis³

Affiliations

¹ Department of Clinical Chemistry, Faculty of Medicine, University of Ioannina, Ioannina, Greece.
² Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
³ Department of Obstetrics and Gynaecology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larisa, Greece.
⁴ Division of Endocrinology, Central Drug Research Institute, Lucknow, India.

Abstract

The broad conceptualization of fertility preservation and restoration has become already a major concern in the modern western world since a large number of individuals often face it in the everyday life. Driven by different health conditions and/or social reasons, a variety of patients currently rely on routinely and non-routinely applied assisted reproductive technologies, and mostly on the possibility to cryopreserve gametes and/or gonadal tissues for expanding their reproductive lifespan. This review embraces the data present in human-focused literature regarding the up-to-date methodologies and tools contemporarily applied in IVF laboratories' clinical setting of the oocyte, sperm, and embryo cryopreservation and explores the latest news and issues related to the optimization of methods used in ovarian and testicular tissue cryopreservation.

Keywords: cryopreservation; embryo; fertility preservation; gametes; gonadal tissue.

Publication types

Review