Digital Holographic Microscopy to Assess Cell Behavior

Brad Bazow; Van K Lam; Thuc Phan; Byung Min Chung; George Nehmetallah; Christopher B Raub

doi:10.1007/978-1-0716-3052-5_16

Digital Holographic Microscopy to Assess Cell Behavior

Methods Mol Biol. 2023:2644:247-266. doi: 10.1007/978-1-0716-3052-5_16.

Authors

Brad Bazow¹, Van K Lam², Thuc Phan¹, Byung Min Chung³, George Nehmetallah¹, Christopher B Raub⁴

Affiliations

¹ Department of Electrical Engineering and Computer Science, The Catholic University of America, Washington, DC, USA.
² Department of Biomedical Engineering, The Catholic University of America, Washington, DC, USA.
³ Department of Biology, The Catholic University of America, Washington, DC, USA.
⁴ Department of Biomedical Engineering, The Catholic University of America, Washington, DC, USA. raubc@cua.edu.

PMID: 37142927
DOI: 10.1007/978-1-0716-3052-5_16

Abstract

Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality checks, selection and setup of imaging chambers, a sampling plan, image acquisition, phase and amplitude map reconstruction, and parameter map post-processing to extract information about cell morphology and/or motility. Each step is described below, focusing on results from imaging four human cell lines. Several post-processing approaches are detailed, with an aim of tracking individual cells and dynamics of cell populations.

Keywords: Cell lines; Cell morphology; Cell motility; Holography; Image processing; Machine learning; Microscopy; Quantitative phase.

MeSH terms

Cell Culture Techniques
Cell Line
Holography*
Humans
Image Interpretation, Computer-Assisted / methods
Microscopy* / methods