High-Efficiency Single-Cell Electrical Impedance Spectroscopy

Yongxiang Feng; Liang Huang; Peng Zhao; Fei Liang; Wenhui Wang

doi:10.1007/978-1-0716-3052-5_6

High-Efficiency Single-Cell Electrical Impedance Spectroscopy

Methods Mol Biol. 2023:2644:81-97. doi: 10.1007/978-1-0716-3052-5_6.

Authors

Yongxiang Feng¹, Liang Huang², Peng Zhao¹, Fei Liang¹, Wenhui Wang³

Affiliations

¹ State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China.
² Anhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, Anhui, China.
³ State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China. wwh@tsinghua.edu.cn.

PMID: 37142917
DOI: 10.1007/978-1-0716-3052-5_6

Abstract

Single-cell impedance measurement is label free and noninvasive in characterizing the electrical properties of single cells. At present, though widely used for impedance measurement, electrical impedance flow cytometry (IFC) and electrical impedance spectroscopy (EIS) are used alone for most microfluidic chips. Here, we describe high-efficiency single-cell electrical impedance spectroscopy, which combines in one chip the IFC and EIS techniques for high-efficiency single-cell electrical property measurement. We envision that the strategy of combining IFC and EIS provides a new thought in the efforts to enhance the efficiency of electrical property measurement for single cells.

Keywords: Electric impedance spectroscopy; Electrical property; Impedance flow cytometry; Microfluidics; Single cell analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dielectric Spectroscopy*
Electric Impedance
Flow Cytometry / methods
Microfluidics / methods
Single-Cell Analysis* / methods