Identification of core cuprotosis-correlated biomarkers in abdominal aortic aneurysm immune microenvironment based on bioinformatics

Jiateng Hu; Song Xue; Zhijue Xu; Zhaoyu Wu; Xintong Xu; Xin Wang; Guang Liu; Xinwu Lu; Bo Li; Xiaobing Liu

doi:10.3389/fimmu.2023.1138126

Identification of core cuprotosis-correlated biomarkers in abdominal aortic aneurysm immune microenvironment based on bioinformatics

Front Immunol. 2023 Apr 17:14:1138126. doi: 10.3389/fimmu.2023.1138126. eCollection 2023.

Authors

Jiateng Hu^{1

2}, Song Xue³, Zhijue Xu^{1

2}, Zhaoyu Wu^{1

2}, Xintong Xu^{1

2}, Xin Wang^{1

2}, Guang Liu^{1

2}, Xinwu Lu^{1

2}, Bo Li^{1

2}, Xiaobing Liu^{1

2}

Affiliations

¹ Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Vascular Centre of Shanghai Jiao Tong University, Shanghai, China.
³ Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Abstract

Background: The occurrence of abdominal aortic aneurysms (AAAs) is related to the disorder of immune microenvironment. Cuprotosis was reported to influence the immune microenvironment. The objective of this study is to identify cuprotosis-related genes involved in the pathogenesis and progression of AAA.

Methods: Differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) in mouse were identified following AAA through high-throughput RNA sequencing. The enrichment analyses of pathway were selected through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG). The validation of cuprotosis-related genes was conducted through immunofluorescence and western blot analyses.

Results: Totally, 27616 lncRNAs and 2189 mRNAs were observed to be differentially expressed (|Fold Change| ≥ 2 and q< 0.05) after AAA, including 10424 up-regulated and 17192 down-regulated lncRNAs, 1904 up-regulated and 285 down-regulated mRNAs. Gene ontology and KEGG pathway analysis showed that the DElncRNAs and DEmRNAs were implicated in many different biological processes and pathways. Furthermore, Cuprotosis-related genes (NLRP3, FDX1) were upregulated in the AAA samples compared with the normal one.

Conclusion: Cuprotosis-related genes (NLRP3,FDX1) involved in AAA immune environment might be critical for providing new insight into identification of potential targets for AAA therapy.

Keywords: Abdominal aortic aneurysm; Cuprotosis; FDX1; NLRP3; immune environment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aortic Aneurysm, Abdominal* / metabolism
Biomarkers
Computational Biology
Gene Expression Profiling
Mice
NLR Family, Pyrin Domain-Containing 3 Protein / genetics
RNA, Long Noncoding* / genetics

Substances

RNA, Long Noncoding
NLR Family, Pyrin Domain-Containing 3 Protein
Biomarkers

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (82170488, 81970405, 81900410, 51890892) and the Natural Science Foundation of Shanghai (21ZR1437300).