Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA

Yuting Sun; Hong Chen; Mengmeng Xu; Liwen He; Hongchen Mao; Shiyao Yang; Xin Qiao; Deqin Yang

doi:10.1080/20002297.2023.2204250

Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA

J Oral Microbiol. 2023 Apr 28;15(1):2204250. doi: 10.1080/20002297.2023.2204250. eCollection 2023.

Authors

Yuting Sun^{1

2

3}, Hong Chen^{1

2

3}, Mengmeng Xu^{1

2

3}, Liwen He^{1

2

3}, Hongchen Mao^{1

2

3}, Shiyao Yang^{1

2

3}, Xin Qiao^{1

2

3}, Deqin Yang^{1

2

3}

Affiliations

¹ Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing, China.
² Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.
³ Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.

Abstract

Background: Streptococcus mutans (S. mutans) is a pivotal cariogenic pathogen contributing to its multiple virulence factors, one of which is synthesizing exopolysaccharides (EPS). VicK, a sensor histidine kinase, plays a major role in regulating genes associated with EPS synthesis and adhesion. Here we first identified an antisense vicK RNA (ASvicK) bound with vicK into double-stranded RNA (dsRNA).

Objective: This study aims to investigate the effect and mechanism of ASvicK in the EPS metabolism and cariogenesis of S. mutans.

Methods: The phenotypes of biofilm were detected by scanning electron microscopy (SEM), gas chromatography-mass spectrometery (GC-MS) , gel permeation chromatography (GPC) , transcriptome analysis and Western blot. Co-immunoprecipitation (Co-ip) assay and enzyme activity experiment were adopted to investigate the mechanism of ASvicK regulation. Caries animal models were developed to study the relationship between ASvicK and cariogenicity of S. mutans.

Results: Overexpression of ASvicK can inhibit the growth of biofilm, reduce the production of EPS and alter genes and protein related to EPS metabolism. ASvicK can adsorb RNase III to regulate vicK and affect the cariogenicity of S. mutans.

Conclusions: ASvicK regulates vicK at the transcriptional and post-transcriptional levels, effectively inhibits EPS synthesis and biofilm formation and reduces its cariogenicity in vivo.

Keywords: Antisense RNA; Streptococcus mutans; bacterial genetics; biofilm growth; dental caries; exopolysaccharides; vicK gene.

Grants and funding

This research was funded by the National Natural Science Foundation of China under Grant Agreement (No. 31970783 and No. 32270888). This work was supported by a grant from National Science Fund for Distinguished Young Scholars (No. 82100991). This research was funded by the Top Talent Distinguished Professor from Chongqing Medical University [No. (2021) 215] and Program for Youth Innovation in Future Medicine from Chongqing Medical University (No. W0060). This research was funded by the Natural Science Foundation Project of Chongqing Science and Technology Commission (cstc2021jcyj-bshX0211) and the Basic Research and Frontiers Exploration Project of Science and Technology Committee of Yuzhong District (No. 20210124).