Assessment of biodegradation kinetics and mass transfer aspects in attached growth bioreactor for effective treatment of brilliant green dye from wastewater

Pranjal Tripathi; Sonam Tiwari; Ravi Kumar Sonwani; Ram Sharan Singh

doi:10.1016/j.biortech.2023.129111

Assessment of biodegradation kinetics and mass transfer aspects in attached growth bioreactor for effective treatment of brilliant green dye from wastewater

Bioresour Technol. 2023 Aug:381:129111. doi: 10.1016/j.biortech.2023.129111. Epub 2023 May 1.

Authors

Pranjal Tripathi¹, Sonam Tiwari², Ravi Kumar Sonwani³, Ram Sharan Singh⁴

Affiliations

¹ Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, Uttar Pradesh, India.
² Department of Civil Engineering, Indian Institute of Technology (BHU), Varanasi 221005, Uttar Pradesh, India.
³ Department of Chemical Engineering, Indian Institute of Petroleum and Energy (IIPE), Visakhapatnam 530003, Andhra Pradesh, India.
⁴ Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, Uttar Pradesh, India. Electronic address: rssingh.che@iithu.ac.in.

PMID: 37137445
DOI: 10.1016/j.biortech.2023.129111

Abstract

In this study, Bacillus licheniformis immobilized with low-density polyethylene (LDPE) was employed to degrade Brilliant Green (BG) dye from wastewater in a packed bed bioreactor (PBBR). Bacterial growth and extracellular polymeric substance (EPS) secretion were also assessed under different concentrations of BG dye. The impacts of external mass transfer resistance on BG biodegradation were also evaluated at different flow rates (0.3-1.2 L/h). A new mass transfer correlation [Formula: see text] was proposed to study the mass transfer aspects in attached-growth bioreactor. The intermediates, namely 3- dimethylamino phenol, benzoic acid, 1-4 benzenediol, and acetaldehyde were identified during the biodegradation of BG and, subsequently degradation pathway was proposed. Han - Levenspiel kinetics parameters μ_max and K_s were found to be 0.185 per day and 115 mg/L, respectively. The new insight into mass transfer and kinetics support the design of efficiently attached growth bioreactor to treat a wide range of pollutants.

Keywords: Attached growth bioreactor; Biodegradation; Bioluminescence assay; Kinetics; Mass transfer coefficient.

MeSH terms

Biodegradation, Environmental
Bioreactors / microbiology
Extracellular Polymeric Substance Matrix / metabolism
Kinetics
Phenol / metabolism
Wastewater*
Water Pollutants, Chemical* / analysis

Substances

Wastewater
brilliant green
Phenol
Water Pollutants, Chemical