Boosting ultralong chemiluminescence for the self-powered time-resolved immunosensor

Aori Qileng; Shizhang Chen; Hongzhi Liang; Mengting Chen; Hongtao Lei; Weipeng Liu; Yingju Liu

doi:10.1016/j.bios.2023.115338

Boosting ultralong chemiluminescence for the self-powered time-resolved immunosensor

Biosens Bioelectron. 2023 Aug 15:234:115338. doi: 10.1016/j.bios.2023.115338. Epub 2023 Apr 20.

Authors

Aori Qileng¹, Shizhang Chen², Hongzhi Liang³, Mengting Chen³, Hongtao Lei⁴, Weipeng Liu³, Yingju Liu⁵

Affiliations

¹ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
² College of Electronic Engineering, South China Agricultural University, Guangzhou, 510642, China.
³ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
⁴ The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
⁵ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address: liuyingju@hotmail.com.

PMID: 37137191
DOI: 10.1016/j.bios.2023.115338

Abstract

The construction of an immunosensor based on ultralong chemiluminescence is challenged due to the shortage of highly efficient initiator for long and stable catalysis. Herein, the heterogeneous Au/Pt@CuO/Cu₂O catalyst was used to investigate the structure-activity relationship, while Au/Pt significantly promotes the activity of CuO/Cu₂O to catalyze H₂O₂ and thus produces ·OH and O₂^•- radicals in highly alkaline solutions, resulting in the strong and long chemiluminescence in the reaction with luminol (10 mL, more than 4 min with 1 μg catalyst). By using the Au/Pt@CuO/Cu₂O as the label in the immunoassay, the strong and long chemiluminescence could initiate the photocurrent of the photoelectrochemical (PEC) substrate, and the luminescence time could influence the photocurrent extinction time, thus a self-powered time-resolved PEC immunosensor was developed to detect furosemide, showing a linear relationship between the extinction time and the logarithm of concentrations from 10^-3 to 1 μg/L. This work not only experimentally verifies that the Pt-O-Cu bond in heterogeneous catalysts breaks the pH limitation of the Fenton reaction, but also realizes the chemiluminescence for self-powered time-resolved immunosensor, thereby expanding the portable applicability of chemiluminescence in food safety inspection, health monitoring, and biomedical detection without external light source.

Keywords: Chemluminescence; Heterogeneous fenton catalyst; Photoelectrochemical; Pt–O–Cu; Self-powered immunosensor.

MeSH terms

Biosensing Techniques* / methods
Electrochemical Techniques / methods
Hydrogen Peroxide
Immunoassay / methods
Limit of Detection
Luminescence*

Substances

cupric oxide
Hydrogen Peroxide