Interplay of silymarin and clove fruit extract effectively enhances cadmium stress tolerance in wheat (Triticum aestivum)

Ahmed H El-Sappah; Mohamed A S Metwally; Mostafa M Rady; Hayssam M Ali; Linghui Wang; Pulak Maitra; Muhammad Ihtisham; Kuan Yan; Xin Zhao; Jia Li; El-Sayed M Desoky

doi:10.3389/fpls.2023.1144319

Interplay of silymarin and clove fruit extract effectively enhances cadmium stress tolerance in wheat (Triticum aestivum)

Front Plant Sci. 2023 Apr 14:14:1144319. doi: 10.3389/fpls.2023.1144319. eCollection 2023.

Authors

Ahmed H El-Sappah^{1

2}, Mohamed A S Metwally³, Mostafa M Rady⁴, Hayssam M Ali⁵, Linghui Wang¹, Pulak Maitra⁶, Muhammad Ihtisham¹, Kuan Yan¹, Xin Zhao¹, Jia Li¹, El-Sayed M Desoky⁷

Affiliations

¹ School of Agriculture, Forestry, and Food Engineering, Yibin University, Yibin, Sichuan, China.
² Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
³ Soil Science Department, Faculty of Agriculture, Zagazig University, Zagazi, Egypt.
⁴ Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
⁵ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
⁶ Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland.
⁷ Botany Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.

Abstract

Introduction: Osmoprotectant supplementation can be used as a useful approach to enhance plant stress tolerance. However, the effect of silymarin and clove fruit extract (CFE) on wheat plants grown under cadmium (Cd) stress has not been studied.

Methods: Wheat seeds were planted in plastic pots filled with ions-free sand. A ½-strength Hoagland's nutrient solution was used for irrigation. Pots were treated with eight treatments thirteen days after sowing: 1) Control, 2) 0.5 mM silymarin foliar application [silymarin], 3) 2% CFE foliar application [CFE], 4) CFE enriched with silymarin (0.24 g silymarin L-1 of CFE) [CFE-silymarin], 5) Watering wheat seedlings with a nutritious solution of 2 mM Cd [Cd]. 6) Cadmium + silymarin, 7) Cadmium + CFE, and 8) Cadmium + CFE-silymarin. The experimental design was a completely randomized design with nine replicates.

Results and discussion: The Cd stress decreased grain yield, shoot dry weight, leaf area, carotenoids, chlorophylls, stomatal conductance, net photosynthetic rate, transpiration rate, membrane stability index, nitrogen, phosphorus, and potassium content by 66.9, 60.6, 56.7, 23.8, 33.5, 48.1, 41.2, 48.7, 42.5, 24.1, 39.9, and 24.1%, respectively. On the other hand, Cd has an Application of CFE, silymarin, or CEF-silymarin for wheat plants grown under Cd stress, significantly improved all investigated biochemical, morphological, and physiological variables and enhanced the antioxidant enzyme activities. Applying CFE and/or silymarin enhanced plant tolerance to Cd stress more efficiently. Our findings suggest using CFE-silymarin as a meaningful biostimulator for wheat plants to increase wheat plants' tolerance to Cd stress via enhancing various metabolic and physiological processes.

Keywords: antioxidant system; heavy metals; osmoprotectants; plant growth regulators; plant stress.

Grants and funding

This work was funded by the Researchers Supporting Project number (RSP2023R123), King Saud University, Riyadh, Saudi Arabia. Also, this work was supported by the Scientific Research Project of Yibin University (Grant numbers 2022QH05 and 2022YY06).