The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells

Chunling Ma; Shanhui Ren; Tadele Berihun Afera; Xue Yang; Yugang Lin; Xiaohong Gao; Fangping Wang; Xusheng Qiu; Xiangwei Wang; Xiangping Yin; Yuefeng Sun; Xuerui Wan; Hao-Tai Chen

doi:10.1016/j.jviromet.2023.114745

The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells

J Virol Methods. 2023 Jul:317:114745. doi: 10.1016/j.jviromet.2023.114745. Epub 2023 Apr 28.

Authors

Affiliations

¹ State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, PR China; Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
² State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, PR China. Electronic address: renshanhui@caas.cn.
³ State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, PR China.
⁴ State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, PR China; Laboratory of Veterinary Microbiology, College of Animal Science and Technology, Tarim University, Alar 843300, PR China.
⁵ Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute. Chinese Academy of Agricultural Science, Shanghai 200241, PR China.
⁶ Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: wanxr@gsau.edu.cn.
⁷ State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, PR China. Electronic address: haotaichen@163.com.

PMID: 37121353
DOI: 10.1016/j.jviromet.2023.114745

Abstract

Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in Asia, including China. Improving the propagation of LSDV is important for diagnostics and vaccine production. Our study identified and compared the LSDV susceptibility of eleven standard cells using western blot, indirect immune-fluorescence assay, quantitative PCR, and 50 % tissue culture infectious dose. Our finding revealed that the LSDV strain could infect five cell lines and show a cytopathic effect. Furthermore, the hTERT-CSF cell line had the highest level of virus in the five cell models, followed by BHK-21, MDBK, Vero, and hTERT-ST. Hence, hTERT-CSF could be used as a candidate cell line for basic and applied research, clinical application, and LSDV vaccine development, providing a vital reference in LSDV and other viruses.

Keywords: Comparative study; HTERT-CSF; Lumpy skin disease virus; Susceptibility.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Asia
Cattle
Cell Line
China
Lumpy Skin Disease*
Lumpy skin disease virus* / genetics
Polymerase Chain Reaction