Multi-mycotoxin determination in plant-based meat alternatives and exposure assessment

Abstract

The aim of this study was to fill in the gap regarding the occurrence of mycotoxins in plant-based meat alternatives. Hence, a multi-mycotoxin method (aflatoxins, ochratoxin A, fumonisins, zearalenone, and mycotoxins from the Alternaria alternata genera) was developed followed by an exposure assessment for the Italian consumers' exposure to mycotoxins. A total of 13 meat alternatives samples based on soy, pea, chickpea, lupin, and seitan were analysed. With the exception of seitan, all of the remaining samples were contaminated with one mycotoxin or mixtures of up to seven mycotoxins. The level of contamination was as low as 0.2 μg/kg alternariol methyl ether and as high as 66.9 μg/kg fumonisin B1. To analyse the exposure to mycotoxins due to plant-based meat alternatives consumption we used the consumption meat data from the Food and Agriculture Organization for Italian adult consumers and simulated a full replacement of meat with plant-based meat alternatives. Based on our model, consumption of plant-based meat alternatives led to a non-tolerable exposure to alternariol (hazard index (HI) > 1) in pea-based burger and soy + wheat-based steak, while samples contaminated with aflatoxins, respectively ochratoxin A, indicated a health concern related to liver and renal cancer (margin of exposure (MOE) < 10,000). This is the first study that presents the co-occurrence of mycotoxins in multiple plant-based meat alternatives. Moreover, these results indicate that there is a need for policymakers to consider the regulation of mycotoxins in plant-based meat alternatives in order to ensure consumers' safety.

Keywords: Exposure assessment; High-performance liquid chromatography; Mass spectrometry; Meat analogue; Mycotoxin co-occurrence; Plant-based meat alternative; Risk characterization; SALLE.