Protective effects of melatonin on deoxynivalenol-induced oxidative stress and autophagy in IPEC-J2 cells

Yafei Xu; Yunxiao Xie; Zhengchang Wu; Haifei Wang; Zhenhai Chen; Jingneng Wang; Wenbin Bao

doi:10.1016/j.fct.2023.113803

Protective effects of melatonin on deoxynivalenol-induced oxidative stress and autophagy in IPEC-J2 cells

Food Chem Toxicol. 2023 Jul:177:113803. doi: 10.1016/j.fct.2023.113803. Epub 2023 Apr 28.

Authors

Yafei Xu¹, Yunxiao Xie², Zhengchang Wu², Haifei Wang², Zhenhai Chen³, Jingneng Wang⁴, Wenbin Bao⁵

Affiliations

¹ Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
² Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
³ Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
⁴ Shanghai Xiongtu Biotechnology Co., Ltd., Shanghai, 200000, China.
⁵ Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: wbbao@yzu.edu.cn.

PMID: 37120089
DOI: 10.1016/j.fct.2023.113803

Abstract

This study explored protective effects of melatonin (MEL) on deoxynivalenol (DON)-induced toxicity in porcine jejunum epithelial cells (IPEC-J2). Cells were preexposed to MEL and then exposed to DON to detect cell viability, apoptosis, and oxidative stress indicators. Compared to DON treatment, pretreatment with MEL significantly increased cell proliferation. (P < 0.01), intracellular catalase (CAT) and superoxide dismutase (SOD) levels (P < 0.05), decreased apoptosis and oxidative stress, and significantly attenuated the inflammatory response. RNA-seq analysis revealed that MEL protects IPEC-J2 from the adverse effects of DON by affecting the expression of tight junction and autophagy pathway-related genes. Similarly, further experiments revealed that MEL partly prevented DON-induced disruption of intestinal barrier function and decreased autophagy induced by DON via activation of the AKT/mTOR pathway. In conclusion, these results demonstrated the preventive properties of MEL against DON-induced cell damage by activating the antioxidant system and Inhibition of autophagy.

Keywords: Autophagy; Deoxynivalenol; Melatonin; Oxidative stress; Tight junction.

MeSH terms

Animals
Autophagy
Cell Line
Epithelial Cells
Melatonin* / pharmacology
Oxidative Stress
Swine

Substances

deoxynivalenol
Melatonin