Potential use of propolis-loaded quaternized chitosan/pectin hydrogel films as wound dressings: Preparation, characterization, antibacterial evaluation, and in vitro healing assay

Abstract

Quaternized chitosan (QCS) was blended with pectin (Pec) to improve water solubility and antibacterial activity of the hydrogel films. Propolis was also loaded into hydrogel films to improve wound healing ability. Therefore, the aim of this study was to fabricate and characterize the propolis-loaded QCS/Pec hydrogel films for use as wound dressing materials. The morphology, mechanical properties, adhesiveness, water swelling, weight loss, release profiles, and biological activities of the hydrogel films were investigated. Scanning Electron Microscope (SEM) investigation indicated a homogenous smooth surface of the hydrogel films. The blending of QCS and Pec increased tensile strength of the hydrogel films. Moreover, the blending of QCS and Pec improved the stability of the hydrogel films in the medium and controlled the release characteristics of propolis from the hydrogel films. The antioxidant activity of the released propolis from the propolis-loaded hydrogel films was ∼21-36 %. The propolis-loaded QCS/Pec hydrogel films showed the bacterial growth inhibition, especially against S. aureus and S. pyogenes. The propolis-loaded hydrogel films were non-toxicity to mouse fibroblast cell line (NCTC clone 929) and supported the wound closure. Therefore, the propolis-loaded QCS/Pec hydrogel films might be good candidates for use as wound dressing materials.

Keywords: Propolis; Quaternized chitosan; Wound dressing.