Staphylococcus aureus Colony Polymerase Chain Reaction

Cold Spring Harb Protoc. 2023 Aug 1;2023(8):107949. doi: 10.1101/pdb.prot107949.

Abstract

Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / metabolism
  • Lysostaphin* / metabolism
  • Peptidoglycan / metabolism
  • Polymerase Chain Reaction
  • Staphylococcus aureus* / genetics
  • Staphylococcus aureus* / metabolism

Substances

  • Lysostaphin
  • Peptidoglycan