Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Lei Wang; Jae-Young Oh; Hye-Won Yang; Jimin Hyun; Ginnae Ahn; Xiaoting Fu; Jiachao Xu; Xin Gao; Seon-Heui Cha; You-Jin Jeon

doi:10.3390/polym15081912

Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Polymers (Basel). 2023 Apr 17;15(8):1912. doi: 10.3390/polym15081912.

Authors

Lei Wang¹, Jae-Young Oh², Hye-Won Yang³, Jimin Hyun³, Ginnae Ahn⁴, Xiaoting Fu¹, Jiachao Xu¹, Xin Gao¹, Seon-Heui Cha⁵, You-Jin Jeon^{3

6}

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
² Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
³ Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
⁴ Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Republic of Korea.
⁵ Department of Marine Bio and Medical Sciences, Hanseo University, Seosan-si 31962, Republic of Korea.
⁶ Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea.

Abstract

Our previous studies have evaluated the bioactivities of a fucoidan isolated from Sargassum fusiforme (SF-F). To further investigate the health benefit of SF-F, in the present study, the protective effect of SF-F against ethanol (EtOH)-induced oxidative damage has been evaluated in in vitro and in vivo models. SF-F effectively improved the viability of EtOH-treated Chang liver cells by suppressing apoptosis. In addition, the in vivo test results indicate that SF-F significantly and dose-dependently increased the survival rate of zebrafish treated with EtOH. Further research results show that this action works through decreasing cell death via reduced lipid peroxidation by scavenging intracellular reactive oxygen species in EtOH-stimulated zebrafish. These results indicate that SF-F effectively protected Chang liver cells and zebrafish against EtOH-induced oxidative damage and suggest the potential of SF-F to be used as an ingredient in the functional food industry.

Keywords: Sargassum fusiforme; ethanol; oxidative stress.

Grants and funding

This research was funded by the Shandong Postdoctoral Science Foundation (SDCX-ZG-202202034), the Qingdao Marine Science and Technology Innovation Project (23-1-3-hygg-23-hy), the Fundamental Research Funds for the Central Universities (202262003), the Qingdao Postdoctoral Applied Research Project (862105040061), the Start-up Fund for Young-talent Program funded by Ocean University of China (862101013159), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1A6A1A03033553).