Genome-Wide Identification, Characterization, and Expression of TCP Genes Family in Orchardgrass

Cheng Wang; Guangyan Feng; Xiaoheng Xu; Linkai Huang; Gang Nie; Dandan Li; Xinquan Zhang

doi:10.3390/genes14040925

Genome-Wide Identification, Characterization, and Expression of TCP Genes Family in Orchardgrass

Genes (Basel). 2023 Apr 16;14(4):925. doi: 10.3390/genes14040925.

Authors

Cheng Wang¹, Guangyan Feng¹, Xiaoheng Xu¹, Linkai Huang¹, Gang Nie¹, Dandan Li¹, Xinquan Zhang¹

Affiliation

¹ College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Abstract

Plant-specific TCP transcription factors regulate several plant growth and development processes. Nevertheless, little information is available about the TCP family in orchardgrass (Dactylis glomerata L.). This study identified 22 DgTCP transcription factors in orchardgrass and determined their structure, phylogeny, and expression in different tissues and developmental stages. The phylogenetic tree classified the DgTCP gene family into two main subfamilies, including class I and II supported by the exon-intron structure and conserved motifs. The DgTCP promoter regions contained various cis-elements associated with hormones, growth and development, and stress responses, including MBS (drought inducibility), circadian (circadian rhythms), and TCA-element (salicylic acid responsiveness). Moreover, DgTCP9 possibly regulates tillering and flowering time. Additionally, several stress treatments upregulated DgTCP1, DgTCP2, DgTCP6, DgTCP12, and DgTCP17, indicting their potential effects regarding regulating responses to the respective stress. This research offers a valuable basis for further studies of the TCP gene family in other Gramineae and reveals new ideas for increasing gene utilization.

Keywords: Dactylis glomerata; TCP gene family; expression analysis; floral development; tillering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dactylis* / genetics
Dactylis* / metabolism
Gene Expression Profiling*
Introns
Phylogeny
Transcription Factors / metabolism

Substances

Transcription Factors

Grants and funding

This research was supported by the Forage Breeding Project of Sichuan Province (2021YFYZ0013), National Natural Science Foundation of China, grant number NSFC 32101422, the earmarked fund for Modern Agro-industry Technology Research System (no. CARS-34), the Sichuan Province’s Science Fund for International Cooperation (2022YFH0058), and the Sichuan Province’s Science Fund for Distinguished Young Scholars under grant (2021JDJQ001).