tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue

Linghui Wang; Hao Gu; Tianci Liao; Yuhang Lei; Yanhao Qiu; Qiuyang Chen; Lei Chen; Shunhua Zhang; Jinyong Wang; Xiaoxia Hao; Dongmei Jiang; Ye Zhao; Lili Niu; Xuewei Li; Linyuan Shen; Mailin Gan; Li Zhu

doi:10.3390/genes14040782

tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue

Genes (Basel). 2023 Mar 23;14(4):782. doi: 10.3390/genes14040782.

Authors

Linghui Wang^{1

2}, Hao Gu^{1

2}, Tianci Liao^{1

2}, Yuhang Lei^{1

2}, Yanhao Qiu^{1

2}, Qiuyang Chen^{1

2}, Lei Chen^{1

2}, Shunhua Zhang^{1

2}, Jinyong Wang³, Xiaoxia Hao^{1

2}, Dongmei Jiang^{1

2}, Ye Zhao^{1

2}, Lili Niu^{1

2}, Xuewei Li^{1

2}, Linyuan Shen^{1

2}, Mailin Gan^{1

2}, Li Zhu^{1

2}

Affiliations

¹ Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China.
² Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
³ Chongqing Academy of Animal Science, Chongqing 402460, China.

Abstract

Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, 20 and 21 of which were particularly expressed in VAT and SAT, respectively, were found in WAT. According to the analysis of the tsRNA/miRNA/mRNA co-expression network, the tsRNAs with differential expression were primarily engaged in the endocrine and immune systems, which fall under the classification of organic systems, as well as the global and overview maps and lipid metropolis, which fall under the category of metabolism. This research also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. This research also discovered that tRF-Gly-GCC-037/tRF-Gly-GCC-042/tRF-Gly-CCC-016 and miR-218a/miR281b may be involved in the regulation of fatty acid metabolism in adipose tissue through SCD based on the tsRNA/miRNA/mRNA/fatty acid network. In conclusion, our findings enrich the understanding of ncRNAs in WAT metabolism and health regulation, as well as reveal the differences between SAT and VAT at the level of tsRNAs.

Keywords: adipose tissue; fatty acid; immunity; pig; tsRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fatty Acids / metabolism
Intra-Abdominal Fat* / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Transfer / genetics
RNA, Transfer / metabolism
Swine / genetics

Substances

MicroRNAs
Fatty Acids
RNA, Transfer
RNA, Messenger

Grants and funding

This research was funded by the National Natural Science Foundation of China: No. 31972524; No. 31902135; National Key Research and Development Program of China: No. 2021YFD1200801; Sichuan Science and Technology Program: No. 2021ZDZX0008, No. 2021YJ0265, No. 2021YFYZ0007, No. scsztd-2022-08-09; China Agriculture Research System of MOF and MARA; National Center of Technology Innovation for Pigs.