Non-spherical gold nanoparticles enhanced fluorescence of carbon dots for norovirus-like particles detection

Abdulhakeem Alzahrani; Tawfiq Alsulami; Ahmad Mohammad Salamatullah; Syed Rahin Ahmed

doi:10.1186/s13036-023-00351-x

Non-spherical gold nanoparticles enhanced fluorescence of carbon dots for norovirus-like particles detection

J Biol Eng. 2023 Apr 27;17(1):33. doi: 10.1186/s13036-023-00351-x.

Authors

Abdulhakeem Alzahrani¹, Tawfiq Alsulami², Ahmad Mohammad Salamatullah², Syed Rahin Ahmed³

Affiliations

¹ Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia. aabdulhakeem@ksu.edu.sa.
² Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia.
³ School of Engineering Practice and Technology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.

Abstract

Background: Norovirus is a common pathogen that causes foodborne outbreaks every year and the increasing number of deaths caused by it has become a substantial concern in both developed and underdeveloped countries. To date, no vaccines or drugs are able to control the outbreak, highlighting the importance of finding specific, and sensitive detection tools for the viral pathogen. Current diagnostic tests are limited to public health laboratories and/or clinical laboratories and are time-consuming. Hence, a rapid and on-site monitoring strategy for this disease is urgently needed to control, prevent and raise awareness among the general public.

Results: The present study focuses on a nanohybridization technique to build a higher sensitivity and faster detection response to norovirus-like particles (NLPs). Firstly, the wet chemical-based green synthesis of fluorescent carbon quantum dots and gold nanoparticles (Au NPs) has been reported. Then, a series of characterization studies were conducted on the synthesized carbon dots and Au NPs, for example, high-resolution transmission emission microscopy, fluorescence spectroscopy, fluorescence life-lime measurement, UV-visible spectroscopy, and X-ray diffraction (XRD). The fluorescence emission of the as-synthesized carbon dots and the absorption of Au NPs were located at 440 nm and 590 nm, respectively. Then, the plasmonic properties of Au NPs were utilized to enhance the fluorescence emission of carbon dots in the presence of NLPs in human serum. Here, the enhanced fluorescence response was linearly correlated up to 1 μg mL^-1. A limit of detection (LOD) value was calculated to be 80.3 pg mL^-1 demonstrating that the sensitivity of the proposed study is 10 times greater than that of the commercial diagnostic kits.

Conclusions: The proposed exciton-plasmon interaction-based NLPs-sensing strategy was highly sensitive, specific, and suitable for controlling upcoming outbreaks. Most importantly, the overall finding in the article will take the technology a step further to applicable point-of-care (POC) devices.

Keywords: Carbon dots; Exciton-plasmon interaction; Fluorescence sensor; Gold nanoparticles; Norovirus-like particles.