Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Ruibo Gao; Rongcai Lu; Xinyao Qiu; Likui Wang; Kun Zhang; Shaoying Wu

doi:10.3390/insects14040388

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Insects. 2023 Apr 17;14(4):388. doi: 10.3390/insects14040388.

Authors

Ruibo Gao^{1

2

3}, Rongcai Lu^{1

2

3}, Xinyao Qiu^{1

2

3}, Likui Wang^{1

2

3}, Kun Zhang^{1

2

3}, Shaoying Wu^{1

2

3}

Affiliations

¹ Sanya Nanfan Research Institute, Hainan University, Yazhou, Sanya 572024, China.
² College of Plant Protection, Hainan University, Haikou 570228, China.
³ Yazhou Bay Science and Technology City, Yazhou, Sanya 572024, China.

Abstract

Pyrethroid resistance of thrips has been reported in many countries, and knockdown resistance (kdr) has been identified as a main mechanism against pyrethroids in many insects. To characterize pyrethroid resistance in Megalurothrips usitatus from the Hainan Province of China, we conducted a biological assay and sequenced the voltage-gated sodium channel gene domain II from M. usitatus field populations. It showed high resistance to the pyrethroids for 2019 and 2020, in which LC₅₀ to lambda-cyhalothrin of M. usitatus was 1683.521 mg/L from Sanya in 2020. The LC₅₀ value of deltamethrin was lower in Haikou than in other locations, which mean the south of Hainan has higher resistance than the north of Hainan. Two mutations of I873S and V1015M were detected in the domain II region of the sodium channel in M. usitatus; however, the mutation frequency of V1015M was only 3.33% and that of I873S was 100%. One is homozygous and the other is a heterozygous mutant type. The three thrips-sensitive strains of sodium channel 873 are highly conserved in amino acids (isoleucine), while the M. usitatus pyrethroid-resistant strains are all serine, so I873S may be related to the resistance of M. usitatus to pyrethroids. The present study will contribute to the understanding of the evolution of pyrethroids resistance and contribute to the development of resistance management of M. usitatus in Hainan.

Keywords: Megalurothrips usitatus; pyrethroids; resistance mutation; voltage-gated sodium channel.

Grants and funding

This research was funded by the Hainan Province Key Research and Development Project (ZDYF2021XDNY190), the Hainan Provincial Natural Science Foundation of China (320RC489), the China National Natural Science Foundation (31960539 and 31901894), the Funding of Hainan University KYQD(ZR) 1963 and KYQD(ZR) 1951, and the Hainan Province Major Scientific and Technological projects (No. ZDKJ202002).