Network pharmacology associated anti-influenza mechanism research of Qingjie-Tuire Granule via STAT1/3 signaling pathway

Yutao Wang; Xin Zhao; Mengjie Xiao; Xiaoying Lin; Qiaolian Chen; Shengle Qin; Huihui Ti; Zifeng Yang

doi:10.1016/j.heliyon.2023.e14649

Network pharmacology associated anti-influenza mechanism research of Qingjie-Tuire Granule via STAT1/3 signaling pathway

Heliyon. 2023 Mar 20;9(3):e14649. doi: 10.1016/j.heliyon.2023.e14649. eCollection 2023 Mar.

Authors

Yutao Wang¹, Xin Zhao², Mengjie Xiao², Xiaoying Lin³, Qiaolian Chen¹, Shengle Qin¹, Huihui Ti⁴, Zifeng Yang^{1

3}

Affiliations

¹ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.
² Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China.
³ Department of Pharmacy, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.
⁴ School of Chinese Medicinal Resource, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

Abstract

Qingjie-Tuire (QT) granule was approved for clinical use and its combination was reported to treat influenza infection. To explore its active component and mechanism, the components of QT granule were retrieved from UPLC-UC-Q-TOF/MS analysis. The genes corresponding to the targets were retrieved using GeneCards and TTD database. The herb-compound-target network was constructed by Cytoscape. The target protein-protein interaction network was built using STRING database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of QT granule to IAV were performed for further study. The regulation to different signaling transduction events and cytokine/chemokine expression of QT granule was evaluated using Western blotting and real-time qPCR. Totally, 47 compounds were identified and effect of QT granule on cell STAT1/3 signaling pathways was confirmed by A549 cell model. The efficiency of QT granule on host cell contributes to its clinical application and mechanism research.

Keywords: Anti-Influenza; Qingjie-Tuire granule; Traditional Chinese medicine.