Effectiveness of near-UVA in SARS-CoV-2 inactivation

Eleonora Frilli; Davide Amodeo; Gabriele Cevenini; Nicola Nante; Gabriele Messina

doi:10.1017/S0950268823000560

Effectiveness of near-UVA in SARS-CoV-2 inactivation

Epidemiol Infect. 2023 Apr 27:151:e76. doi: 10.1017/S0950268823000560.

Authors

Eleonora Frilli¹, Davide Amodeo², Gabriele Cevenini³, Nicola Nante^{1

2}, Gabriele Messina^{1

2}

Affiliations

¹ Post-Graduate School of Public Health, University of Siena, Siena, Italy.
² Hygiene and Preventive Medicine, University of Siena, Siena, Italy.
³ Department of Medical Biotechnologies, University of Siena, Siena, Italy.

Abstract

This experimental study aimed to determine the activity of a near-UVA (405 nm) LED ceiling system against the SARS-CoV-2 virus. The ceiling system comprised 17 near-UVA LED lights with a radiant power of 1.1 W/each centred at 405 nm wavelength. A 96-multiwell plate, fixed to a wooden base, was inoculated with suspensions of VERO E6 cell cultures infected with SARS-CoV-2 virus and irradiated at a distance of 40 cm with a dose of 20.2 J/cm² for 120 min. The collected suspensions were transferred to VERO cell culture plates and incubated for 3 days. The maximum measurable log reduction obtained, starting from a concentration of 10^7.2 TCID50/mL, was 3.0 log₁₀ and indicated inhibition of SARS-CoV-2 replication by the near-UVA LED ceiling system. Near-UVA light at a 405-nm wavelength is emerging as a potential alternative treatment for localised infections and environmental decontamination because it is far less harmful to living organisms' cells than UV-C irradiation.

Keywords: COVID-19; Cell Colture; Disinfection; Hygiene; Prevention; SARS-CoV-2; UVC; near-UVA; virus infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
COVID-19*
Chlorocebus aethiops
SARS-CoV-2*
Suspensions
Ultraviolet Rays*
Vero Cells

Substances

Suspensions