A novel RhB@MOF-808 fluorescent probe for the rapid detection of dopamine and Fe3

Abstract

Dopamine (DA) and Fe³⁺ as the important bioactive ingredients, playing an indispensable role in human metabolism. Therefore, developing the accurate detection of DA and Fe³⁺ is of great significance for disease screening. Herein, we put forward a simple, rapid, and sensitive fluorescent detection strategy for the detection of dopamine and Fe³⁺ based on Rhodamine B-modified MOF-808 (RhB@MOF-808). RhB@MOF-808 produced strong fluorescence at 580 nm, and the fluorescence was significantly quenched after DA or Fe³⁺ was added, which was regarded as a static quenching process. Detection limits are as low as 60.25 nM and 48.34 nM, respectively. Furthermore, based on the responses of DA and Fe³⁺ to the probe, molecular logic gates were successfully designed. More importantly, RhB@MOF-808 had excellent cell membrane permeability and had been successfully used to label DA and Fe³⁺ in Hela cells, which presented a potential biological application value as a fluorescent probe for detecting DA and Fe³⁺.

Keywords: Dopamine; Fe(3+); Fluorescent; MOF-808.