Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

Appl Biochem Biotechnol. 2024 Jan;196(1):85-98. doi: 10.1007/s12010-023-04482-1. Epub 2023 Apr 26.

Abstract

New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range of consumer items, are of significant interest. Hence, this research emphasizes the biological approach of Ag-NPs through Egyptian henna leaves (Lawsonia inermis Linn.) extracts and analysis of the prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses of prepared Ag-NPs were carried out through UV-visible (UV-Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. UV-Vis reveals that Ag-NPs have a maximum peak at 460 nm in visible light. Structural characterization recorded peaks that corresponded to Bragg's diffractions for silver nano-crystal, with average crystallite sizes varying from 28 to 60 nm. Antibacterial activities of Ag-NPs were examined, and it is observed that all microorganisms are very sensitive to biologically synthesized Ag-NPs.

Keywords: Antibacterial activity; Lawsonia inermis Linn.; Silver nanoparticles; Urinary tract infection.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Lawsonia Plant*
  • Metal Nanoparticles* / chemistry
  • Microbial Sensitivity Tests
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Silver / chemistry
  • Silver / pharmacology
  • Spectroscopy, Fourier Transform Infrared
  • Urinary Tract Infections*
  • X-Ray Diffraction

Substances

  • Silver
  • Anti-Bacterial Agents
  • Plant Extracts