Effect of hydrochar on antibiotic-resistance genes and relevant mechanisms during chicken manure composting

Guangchun Shan; Jie Liu; Bin Zhu; Wenbing Tan; Weiguang Li; Zhurui Tang; Xinhao Hu; Lin Zhu; Beidou Xi

doi:10.1016/j.jhazmat.2023.131459

Effect of hydrochar on antibiotic-resistance genes and relevant mechanisms during chicken manure composting

J Hazard Mater. 2023 Jul 15:454:131459. doi: 10.1016/j.jhazmat.2023.131459. Epub 2023 Apr 20.

Authors

Guangchun Shan¹, Jie Liu², Bin Zhu³, Wenbing Tan⁴, Weiguang Li⁵, Zhurui Tang⁶, Xinhao Hu⁷, Lin Zhu², Beidou Xi⁷

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; State Key Laboratory of Environmental Criteria and Risk Assessment, and State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
² State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
³ Shenergy Environmental Technologies Co., LTD, Hangzhou 311100, China.
⁴ State Key Laboratory of Environmental Criteria and Risk Assessment, and State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: wenbingtan@126.com.
⁵ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: hitlwg@126.com.
⁶ College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.
⁷ State Key Laboratory of Environmental Criteria and Risk Assessment, and State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

PMID: 37094443
DOI: 10.1016/j.jhazmat.2023.131459

Abstract

The reduction of enhanced antibiotic resistance genes (ARGs) in compost is important to mitigate the risk of ARG transmission in agricultural production. Hydrochar is used in many applications as a functional carbon material with adsorption and catalytic properties. This study investigated the effects of hydrochar addition on bacterial communities, mobile genetic elements (MGEs), and ARGs in chicken manure composting. The addition of 2%, 5%, and 10% hydrochar (dry weight) reduced the total numbers of target ARGs and MGEs in the compost products by 40.13-55.33% and 23.63-37.23%, respectively. Hydrochar changed the succession of the bacterial population during composting, lowering the abundance of potential pathogens and promoting microbial activity in amino acid and carbohydrate metabolism. A significant possible microbial host for ARGs was found to be Firmicutes. Hydrochar was found to affect the host microorganisms and MGEs directly by altering environmental factors that indirectly impacted the ARG profiles, as shown by partial least squares pathway modeling analysis. In conclusion, the addition of hydrochar to compost is a simple and effective method to promote the removal of ARGs.

Keywords: ARGs; Bacteria communities; Environmental factors; Host microbes; Mobile genetic elements.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacteria / genetics
Chickens
Composting*
Drug Resistance, Microbial / genetics
Genes, Bacterial*
Manure / microbiology

Substances

Manure
Anti-Bacterial Agents