Taxonomic revision of Termitomyces species found in Ryukyu Archipelago, Japan, based on phylogenetic analyses with three loci

Yuuki Kobayashi; Miyuki Katsuren; Masaru Hojo; Shohei Wada; Yoshie Terashima; Masayoshi Kawaguchi; Gaku Tokuda; Kazuhiko Kinjo; Shuji Shigenobu

doi:10.47371/mycosci.2021.11.001

Taxonomic revision of Termitomyces species found in Ryukyu Archipelago, Japan, based on phylogenetic analyses with three loci

Mycoscience. 2022 Jan 20;63(1):33-38. doi: 10.47371/mycosci.2021.11.001. eCollection 2022.

Authors

Yuuki Kobayashi¹, Miyuki Katsuren², Masaru Hojo^{1

3

4

5}, Shohei Wada⁶, Yoshie Terashima³, Masayoshi Kawaguchi^{7

8}, Gaku Tokuda³, Kazuhiko Kinjo², Shuji Shigenobu^{1

8

9}

Affiliations

¹ a Laboratory of Evolutionary Genomics, National Institute for Basic Biology.
² b Department of Bioscience and Biotechnlogy, Faculty of Agriculture, University of the Ryukyus.
³ c Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus.
⁴ e Department of Environmental Sciences and Technology, Faculty of Agriculture, University of the Ryukyus.
⁵ f Global Education Institute, University of the Ryukyus.
⁶ g Faculty of Environment and Information Studies, Keio University.
⁷ d Division of Symbiotic systems, National Institute for Basic Biology.
⁸ h Department of Basic Biology, SOKENDAI.
⁹ i Functional Genomics Facility, National Institute for Basic Biology.

Abstract

Fungi in the genus Termitomyces are external symbionts of fungus-growing termites. The three rhizogenic Termitomyces species T. eurrhizus, T. clypeatus, and T. intermedius, and one species similar to T. microcarpus that lacks pseudorrhiza, have been reported from Ryukyu Archipelago, Japan. In contrast, only two genetic groups (types A and B) of Termitomyces vegetative mycelia have been detected in nests of the fungus-growing termite Odontotermes formosanus. In this study, we investigated the relationships between the mycelial genetic groups and the basidiomata of Termitomyces samples from the Ryukyu Archipelago. We found that all the basidioma specimens and the type B mycelia formed one clade that we identified as T. intermedius. Another clade consisted of the type A mycelia, which showed similarity to T. microcarpus, was identified as T. fragilis. Our results indicate that the Japanese T. eurrhizus and T. clypeatus specimens should re-named as T. intermedius.

Keywords: Lyophyllaceae; symbiosis; termitophillic fungi.

2022, by The Mycological Society of Japan.