Gerhardtia tomentosa and Ossicaulis borealis (Agaricales, Lyophyllaceae)-Two new species from northeast China

Yue Qi; Fei Xu; Zhao-Qian Yang; Jian-Xuan Hou; Ai-Guo Xu; Hong-Bo Guo; Xiao-Dan Yu; Rui-Heng Yang

doi:10.3389/fmicb.2023.1118853

Gerhardtia tomentosa and Ossicaulis borealis (Agaricales, Lyophyllaceae)-Two new species from northeast China

Front Microbiol. 2023 Apr 5:14:1118853. doi: 10.3389/fmicb.2023.1118853. eCollection 2023.

Authors

Yue Qi¹, Fei Xu¹, Zhao-Qian Yang¹, Jian-Xuan Hou¹, Ai-Guo Xu², Hong-Bo Guo³, Xiao-Dan Yu¹, Rui-Heng Yang⁴

Affiliations

¹ College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
² Alpine Fungarium, Tibet Plateau Institute of Biology, Lhasa, China.
³ College of Life Engineering, Shenyang Institute of Technology, Fushun, China.
⁴ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Abstract

Background: Gerhardtia and Ossicaulis are two genera within the family Lyophyllaceae, which show an apparently poor species diversity worldwide. During the field investigation on wild macrofungi, six interesting collections within Gerhardtia and Ossicaulis genera are discovered in the northeastern China.

Methods: To identify whether these collections of Gerhardtia and Ossicaulis are novel species, we performed phylogenetic analyzes using the following DNA regions: the internal transcribed spacer (ITS) region and the large subunit nuclear ribosomal RNA (nrLSU) region. Moreover, a traditional morphological method also be conducted based on both the macro-morphological and micro-morphological features.

Results: The results indicated that these collections tested formed two independent lineages in each genus with a high support. In addition, they can easily be separated from all other taxa of the two genera in morphology. Based on the combination of morphological and molecular data, Gerhardtia tomentosa and Ossicaulis borealis, are confirmed as two new species to science.

Discussions: This study provided a theoretical basis is for the two lyophylloid genera and indicated that the biodiversity resources of northeastern China might be underestimated.

Keywords: Gerhardtia; Lyophyllaceae; Ossicaulis; morphology; phylogeny; taxonomy.

Grants and funding

This research was funded by the Shanghai Action Plan for Scientific and Technological Innovation (20310741800 and 19391901400), the Science and Technology Plan Project of Liaoning Province (2020-MZLH-33), Key Research and Development Plan of Tibet (XZ202201ZY0010N), the National Natural Science Foundation of China (No. 31770014), and the research and development of wild macrofungal germplasm resources collection and preservation technology in Tibet Germplasm Resource Bank (ZDZ × 2018000017).