Bioremediation system consisted with Leclercia adecarboxylata and nZVI@Carbon/Phosphate for lead immobilization: The passivation mechanisms of chemical reaction and biological metabolism in soil

Zedong Teng; Xin Zhao; Bojie Jia; Liangjun Ye; Shaojing Tian; Huiyuan Guo; Yali Guo; Xiaonan Ji; Tinggang Li; Min Li

doi:10.1016/j.jenvman.2023.117888

Bioremediation system consisted with Leclercia adecarboxylata and nZVI@Carbon/Phosphate for lead immobilization: The passivation mechanisms of chemical reaction and biological metabolism in soil

J Environ Manage. 2023 Aug 15:340:117888. doi: 10.1016/j.jenvman.2023.117888. Epub 2023 Apr 21.

Authors

Zedong Teng¹, Xin Zhao², Bojie Jia³, Liangjun Ye⁴, Shaojing Tian⁴, Huiyuan Guo³, Yali Guo⁵, Xiaonan Ji⁵, Tinggang Li⁶, Min Li⁷

Affiliations

¹ Key Laboratory of Green Process and Engineering, Beijing Engineering Research Centre of Process Pollution Control, National Engineering Research Center of Green Recycling for Strategic Metal Resources, Institute of Process Engineering, Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China; Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China. Electronic address: zdteng@ipe.ac.cn.
² Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China.
³ Key Laboratory of Green Process and Engineering, Beijing Engineering Research Centre of Process Pollution Control, National Engineering Research Center of Green Recycling for Strategic Metal Resources, Institute of Process Engineering, Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China.
⁴ Ganjiang Innovation Academy, Jiangxi Province Key Laboratory of Cleaner Production of Rare Earths, Chinese Academy of Sciences, Ganzhou, 341000, China.
⁵ Shanghai Investigation, Design & Research Institute Co., Ltd., Shanghai, 200335, China; YANGTZE Eco-Environment Engineering Research Center (Shanghai), China Three Gorges Corporation, Shanghai, 200335, China.
⁶ Key Laboratory of Green Process and Engineering, Beijing Engineering Research Centre of Process Pollution Control, National Engineering Research Center of Green Recycling for Strategic Metal Resources, Institute of Process Engineering, Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China; Ganjiang Innovation Academy, Jiangxi Province Key Laboratory of Cleaner Production of Rare Earths, Chinese Academy of Sciences, Ganzhou, 341000, China.
⁷ Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China. Electronic address: minli@bjfu.edu.cn.

PMID: 37087891
DOI: 10.1016/j.jenvman.2023.117888

Abstract

Bioremediation is one of the most promising strategies for heavy metal immobilization. A new remediation system was demonstrated in this research, which combined phosphate solubilizing bacteria (PSB) with nZVI@Carbon/Phosphate (nZVI@C/P) composite to remediate lead contaminated soil. Experimental results indicated that the new system (nZVI@C/P + PSB) could effectively convert the labile Pb into the stable fraction after 30 days of incubation, which increased the maximum residual fraction percentage of Pb by 70.58%. The characterization results showed that lead may exist in the forms of Pb₅(PO₄)₃Cl, PbSO₄ and 3PbCO₃·2Pb(OH)₂·H₂O in the soil treated with nZVI@C/P + PSB. Meanwhile, soil enzyme activities and Leclercia abundance were enhanced in the treated soil compared with CK during the incubation time. In addition, the specialized functions (e.g. ABC transporters, siderophore metabolism, sulfur metabolism and phosphorus metabolism) in PSB and nZVI@C/P + PSB group were also enhanced. These phenomena proved that the key soil metabolic functions may be maintained and enhanced through the synergistic effect of incubated PSB and nZVI@C/P. The study demonstrated that this new bioremediation system provided feasible way to improve the efficacy for lead contaminated soil remediation.

Keywords: Lead fraction; PSB; Passivation; Soil metabolic function; nZVI@Carbon/phosphate.

MeSH terms

Bacteria / metabolism
Biodegradation, Environmental
Carbon / metabolism
Enterobacteriaceae
Environmental Restoration and Remediation*
Lead
Phosphates / chemistry
Soil / chemistry
Soil Pollutants* / chemistry

Substances

Phosphates
Carbon
Lead
Soil
Soil Pollutants

Supplementary concepts

Leclercia adecarboxylata