CRISPR-Cas9-mediated editing of the OsHPPD 3' UTR confers enhanced resistance to HPPD-inhibiting herbicides in rice

Yunyu Wu; Ning Xiao; Yue Cai; Qian Yang; Ling Yu; Zichun Chen; Wei Shi; Jianju Liu; Cunhong Pan; Yuhong Li; Xiaoxiang Zhang; Changhai Zhou; Niansheng Huang; Hongjuan Ji; Shuhao Zhu; Aihong Li

doi:10.1016/j.xplc.2023.100605

CRISPR-Cas9-mediated editing of the OsHPPD 3' UTR confers enhanced resistance to HPPD-inhibiting herbicides in rice

Plant Commun. 2023 Sep 11;4(5):100605. doi: 10.1016/j.xplc.2023.100605. Epub 2023 Apr 23.

Authors

Yunyu Wu¹, Ning Xiao¹, Yue Cai¹, Qian Yang¹, Ling Yu¹, Zichun Chen¹, Wei Shi¹, Jianju Liu¹, Cunhong Pan¹, Yuhong Li¹, Xiaoxiang Zhang¹, Changhai Zhou¹, Niansheng Huang¹, Hongjuan Ji¹, Shuhao Zhu¹, Aihong Li²

Affiliations

¹ Institute of Agricultural Sciences for Lixiahe Region in Jiangsu, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, China.
² Institute of Agricultural Sciences for Lixiahe Region in Jiangsu, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, China. Electronic address: yzlah@126.com.

Abstract

This study reports the creation of herbicide-resistant rice lines via CRISPR-Cas9-mediated editing of the 3' UTR of OsHPPD. Resistance index calculations revealed that two resistant lines, TS8-2^#-10 and TS8-8^#-6, exhibited 4.8-fold and 3.7-fold greater resistance to HPPD-inhibiting herbicides compared with the wild type, YG3012.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
CRISPR-Cas Systems / genetics
Herbicides* / pharmacology
Oryza* / genetics

Substances

3' Untranslated Regions
Herbicides