Generation of a human induced pluripotent stem cell line from a pediatric heart failure patient with de novo DNM1L mutation

Anshuman Sinha; Sameer Ahmad Guru; Shankara Varadarajan; Angel Alvarez; Sunjay Kaushal; Xuebin Fu

doi:10.1016/j.scr.2023.103092

Generation of a human induced pluripotent stem cell line from a pediatric heart failure patient with de novo DNM1L mutation

Stem Cell Res. 2023 Jun:69:103092. doi: 10.1016/j.scr.2023.103092. Epub 2023 Apr 12.

Authors

Anshuman Sinha¹, Sameer Ahmad Guru¹, Shankara Varadarajan¹, Angel Alvarez², Sunjay Kaushal³, Xuebin Fu⁴

Affiliations

¹ Department of Cardiovascular-Thoracic Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Department of Pediatrics, Ann & Robert H. Lurie Children's Hospital, Chicago, IL, USA.
² Center for Stem Cell Biology & Regenerative Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
³ Department of Cardiovascular-Thoracic Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Department of Pediatrics, Ann & Robert H. Lurie Children's Hospital, Chicago, IL, USA. Electronic address: skaushal@luriechildrens.org.
⁴ Department of Cardiovascular-Thoracic Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Department of Pediatrics, Ann & Robert H. Lurie Children's Hospital, Chicago, IL, USA. Electronic address: xfu@luriechildrens.org.

PMID: 37086582
DOI: 10.1016/j.scr.2023.103092

Abstract

We generated a new iPSC line (LCHi003-A) from the pediatric dilated cardiomyopathy patient carrying the de novo mutation on DNM1L gene. The new iPSC line expressed high pluripotent markers and were capable to differentiate into trilineage.

MeSH terms

Cardiomyopathy, Dilated* / genetics
Child
Dynamins / metabolism
Heart Failure* / genetics
Humans
Induced Pluripotent Stem Cells* / metabolism
Mutation / genetics

Substances

DNM1L protein, human
Dynamins