Optimizing the synthesis of yeast Beta-glucan via response surface methodology for nanotechnology application

Alshimaa A Atta-Allah; Rania F Ahmed; Azza A M Shahin; Enas A Hassan; Heba Abd-Alla El-Bialy; Mohie Z El-Fouly

doi:10.1186/s12866-023-02845-6

Optimizing the synthesis of yeast Beta-glucan via response surface methodology for nanotechnology application

BMC Microbiol. 2023 Apr 20;23(1):110. doi: 10.1186/s12866-023-02845-6.

Authors

Alshimaa A Atta-Allah¹, Rania F Ahmed², Azza A M Shahin¹, Enas A Hassan², Heba Abd-Alla El-Bialy³, Mohie Z El-Fouly¹

Affiliations

¹ Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
² Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
³ Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt. elbialyheba@yahoo.com.

Abstract

Background: The production of biopolymers from waste resources is a growing trend, especially in high-population countries like Egypt. Beta-glucan (β-glucan) belongs to natural polysaccharides that are derived from plant and microbial origins. In this study, following increasing demands for β-glucan owing to its bioactive properties, a statistical model to enhance microbial β-glucan production was evaluated for its usefulness to the food and pharmaceutical industries. In addition, a trial to convert β-glucan polymer to nanostructure form was done to increase its bioactivity.

Results: Ingredients of low-cost media based on agro-industrial wastes were described using Plackett-Burman and central composite design of response surface methodology for optimizing yeast β-glucan. Minerals and vitamin concentrations significantly influenced β-glucan yield for Kluyveromyces lactis and nitrogen and phosphate sources for Meyerozyma guilliermondii. The maximum predicted yields of β-glucan recovered from K. lactis and M. guilliermondii after optimizing the medium ingredients were 407 and 1188 mg/100 ml; respectively. For the first time, yeast β-glucan nanoparticles (βGN) were synthesized from the β-glucan polymer using N-dimethylformamide as a stabilizer and characterized using UV-vis spectroscopy, transmission electron microscope (TEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The average size of βGN was about 300 nm as determined by DLS. The quantitative variation of functional groups between β-glucan polymer and βGN was evaluated by FT-IR for explaining the difference in their biological activity against Normal Homo sapiens-Hela contaminant and Hepatic cancer cell lines.

Conclusions: Enriching the low-cost media based on agro-industrial wastes with nutritional ingredients improves the yield of yeast β-glucan. The present study succeeds to form β-glucan nanoparticles by a simple method.

Keywords: Agro-industrial wastes; Kluyveromyces lactis; Meyerozyma guilliermondii; Plackett–Burman; Response surface methodology; β-glucan; β-glucan nanoparticles (βGN).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Industrial Waste
Nanoparticles* / chemistry
Nanotechnology
Spectroscopy, Fourier Transform Infrared
beta-Glucans* / chemistry
beta-Glucans* / metabolism

Substances

beta-Glucans
Industrial Waste