Fe3O4/ZIFs-based magnetic solid-phase extraction for the effective extraction of two precursors with diverse structures in aflatoxin B1 biosynthetic pathway

Yaqi Zhang; Yong Man; Jingna Li; Yaming Sun; Xiuming Jiang; Lijun He; Shusheng Zhang

doi:10.1016/j.talanta.2023.124534

Fe₃O₄/ZIFs-based magnetic solid-phase extraction for the effective extraction of two precursors with diverse structures in aflatoxin B₁ biosynthetic pathway

Talanta. 2023 Jul 1:259:124534. doi: 10.1016/j.talanta.2023.124534. Epub 2023 Apr 7.

Authors

Yaqi Zhang¹, Yong Man², Jingna Li², Yaming Sun², Xiuming Jiang², Lijun He³, Shusheng Zhang⁴

Affiliations

¹ School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, PR China; SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, PR China.
² School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, PR China.
³ School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, PR China. Electronic address: lijunhe@haut.edu.cn.
⁴ Center for Modern Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, 450001, PR China.

PMID: 37080071
DOI: 10.1016/j.talanta.2023.124534

Abstract

The aflatoxin B₁ (AFB₁) early warning technique based on precursors is an effective strategy for the prevention of AFB₁ contamination risk. The determination of precursors is imperative to ensure the efficiency of the early warning technique. Herein, a controllable magnetic adsorbent Fe₃O₄/ZIFs was first introduced for the effective extraction and determination of averantin (AVN) and sterigmatocystin (ST) precursors in cereal by combining magnetic solid-phase extraction (MSPE) and high-performance liquid chromatography (HPLC). Benefiting from the abundant adsorption sites and multifunctional groups matching the analytes, Fe₃O₄/ZIFs effectively and simultaneously extracted AVN and ST with great differences in polarity and structure via multiple interactions. AVN was extracted by Fe₃O₄/ZIFs mainly through π-π and hydrophobic interactions, while ST was extracted predominantly by electrostatic interactions and surface complexation. The limits of detection were 0.08 μg kg^-1 (AVN) and 0.36 μg kg^-1 (ST). The developed method exhibited satisfactory spiked recoveries (79.1%-105.4%) in the determination of AVN and ST in rice. This work provides a novel analytical strategy for further studying AFB₁ early warning technique and the formation and transformation of aflatoxins.

Keywords: AFB(1); Extraction mechanism; Fe(3)O(4)/ZIFs adsorbent; Magnetic solid-phase extraction; Precursor.

MeSH terms

Aflatoxin B1 / biosynthesis
Aflatoxin B1 / chemistry
Chromatography, High Pressure Liquid
Magnetic Fields
Oryza* / chemistry
Oryza* / metabolism
Reproducibility of Results
Solid Phase Extraction

Substances

Aflatoxin B1
ferric oxide