Isolation and Mass Spectrometry-Based Profiling of Major Lipids in Brown Adipose Tissue

Dongliang Lu; Hideji Fujiwara; Irfan J Lodhi; Fong-Fu Hsu

doi:10.1007/978-1-0716-3167-6_20

Isolation and Mass Spectrometry-Based Profiling of Major Lipids in Brown Adipose Tissue

Methods Mol Biol. 2023:2662:219-239. doi: 10.1007/978-1-0716-3167-6_20.

Authors

Dongliang Lu¹, Hideji Fujiwara¹, Irfan J Lodhi¹, Fong-Fu Hsu²

Affiliations

¹ Division of Endocrinology, Metabolism and Lipid Research, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA.
² Division of Endocrinology, Metabolism and Lipid Research, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA. fhsu@wustl.edu.

PMID: 37076685
DOI: 10.1007/978-1-0716-3167-6_20

Abstract

Brown adipose tissue (BAT) is an important regulator of metabolic homeostasis through its role in adaptive thermogenesis and control of whole-body glucose metabolism. Lipids play multiple roles in BAT functions, including serving as a fuel source for thermogenesis, mediating inter-organelle cross talk, and acting as BAT-derived signaling molecules that influence systemic energy metabolism. Profiling of various lipids in BAT under distinct metabolic states could provide new insights into their roles in the biology of the thermogenic fat. In this chapter, we describe a step-by-step workflow starting from sample preparations to mass spectrometry-based analysis of fatty acids and phospholipids in BAT.

Keywords: Brown adipose tissue; Folch method; Free fatty acid; Mass spectrometry; Phospholipid; Solid phase extraction; Sphingolipid.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adipose Tissue, Brown* / metabolism
Energy Metabolism*
Fatty Acids / metabolism
Homeostasis
Thermogenesis

Substances

Fatty Acids

Abstract

Publication types

MeSH terms

Substances

Grants and funding