Skin temperature and skin humidity are used for monitoring physiological processes, such as respiration. Despite advances in wearable temperature and humidity sensors, the fabrication of a durable and sensitive sensor for practical uses continues to pose a challenge. Here, we developed a durable, sensitive, and wearable temperature and humidity sensor. A reduced graphene oxide (rGO)/silk fibroin (SF) sensor was fabricated by employing a layer-by-layer technique and thermal reduction treatment. Compared with rGO, the elastic bending modulus of rGO/SF could be increased by up to 232%. Furthermore, an evaluation of the performance of an rGO/SF sensor showed that it had outstanding robustness: it could withstand repeatedly applied temperature and humidity loads and repeated bending. The developed rGO/SF sensor is promising for practical applications in healthcare and biomedical monitoring.