Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis

Junwei Hou; Shuanglian Wang; Rui Miao; Xin Zhang; Mien-Chie Hung

doi:10.1007/978-1-0716-3040-2_11

Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis

Methods Mol Biol. 2023:2641:135-146. doi: 10.1007/978-1-0716-3040-2_11.

Authors

Junwei Hou^{1

2

3

4

5

6}, Shuanglian Wang^{3

4}, Rui Miao^{1

3

4}, Xin Zhang^{1

2

3

4

7}, Mien-Chie Hung^{8

9

10

11}

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
² Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
³ Xiangya Cancer Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁴ National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁵ Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁶ Clinical Research Center for Breast Cancer In Hunan Province, Changsha, Hunan, China.
⁷ Clinical Research Center for Pharyngolaryngeal Diseases and Voice Disorders in Hunan Province, Changsha, Hunan, China.
⁸ Clinical Research Center for Breast Cancer In Hunan Province, Changsha, Hunan, China. mhung@cmu.edu.tw.
⁹ Graduate Institute of Biomedical Sciences, Research Center for Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan. mhung@cmu.edu.tw.
¹⁰ Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. mhung@cmu.edu.tw.
¹¹ Department of Biotechnology, Asia University, Taichung, Taiwan. mhung@cmu.edu.tw.

PMID: 37074647
DOI: 10.1007/978-1-0716-3040-2_11

Abstract

Gasdermin proteins except DFNB59 are the executioners of pyroptotic cell death. Cleavage of a gasdermin by an active protease causes lytic cell death. Gasdermin C (GSDMC) is cleaved by caspase-8 in response to macrophage-secreted TNF-α. Upon cleavage, the GSDMC-N domain is liberated and oligomerized, followed by pore formation in the plasma membrane. GSDMC cleavage, LDH release, and plasma membrane translocation of GSDMC-N domain are the reliable markers for GSDMC-mediated cancer cell pyroptosis (CCP). Here, we describe the methods for examining GSDMC-mediated CCP.

Keywords: Cancer cell; GSDMC; Gasdermin C; HeLa; LDH; Pyroptosis; TNF-α.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Death
Gasdermins
Inflammasomes / metabolism
Intracellular Signaling Peptides and Proteins / metabolism
Neoplasm Proteins / metabolism
Neoplasms*
Pyroptosis*

Substances

Gasdermins
Neoplasm Proteins
Intracellular Signaling Peptides and Proteins
Inflammasomes

Grants and funding

P30 CA016672/CA/NCI NIH HHS/United States