Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus

Qiwen Bao; Gang Li; Zhengchun Yang; Jun Liu; Hanjie Wang; Gaoju Pang; Qianjin Guo; Jun Wei; Wenbo Cheng; Ling Lin

doi:10.1007/s11581-023-04944-w

Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus

Ionics (Kiel). 2023;29(5):2021-2029. doi: 10.1007/s11581-023-04944-w. Epub 2023 Mar 9.

Authors

Qiwen Bao¹, Gang Li¹, Zhengchun Yang², Jun Liu², Hanjie Wang³, Gaoju Pang³, Qianjin Guo⁴, Jun Wei⁵, Wenbo Cheng⁶, Ling Lin¹

Affiliations

¹ School of Precision Instrument and Optoelectronic Engineering, the State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, 92 Weijin Road, Tianjin, 300072 China.
² School of Electrical and Electronic Engineering, Tianjin Key Laboratory of Film Electronic & Communication Devices, Advanced Materials and Printed Electronics Center, Tianjin University of Technology, Tianjin, 300384 China.
³ School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Tianjin University, 92 Weijin Road, Tianjin, 300072 China.
⁴ Analysis and Testing Center, Tianjin University, 92 Weijin Road, Tianjin, 300072 China.
⁵ School of Materials Science and Engineering, Harbin Institute of Technology, Shenzhen, 518055 China.
⁶ Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163 People's Republic of China.

Abstract

To cope with the easy transmissibility of the avian influenza A virus subtype H1N1, a biosensor was developed for rapid and highly sensitive electrochemical immunoassay. Based on the principle of specific binding between antibody and virus molecules, the active molecule-antibody-adapter structure was formed on the surface of an Au NP substrate electrode; it included a highly specific surface area and good electrochemical activity for selective amplification detection of the H1N1 virus. The electrochemical test results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode was used for the electrochemical detection of the H1N1 virus with a sensitivity of 92.1 µA (pg/mL)^-1 cm², LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of (R ² = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for the molecular detection of the H1N1 virus will be of great use in the field of epidemic prevention and raw poultry protection.

Supplementary information: The online version contains supplementary material available at 10.1007/s11581-023-04944-w.

Keywords: Au NPs; H1N1 virus; Immunoassay; Virus sensor.

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