Analysis of Salmonella lineage-specific traits upon cell sorting

Front Cell Infect Microbiol. 2023 Mar 29:13:1146070. doi: 10.3389/fcimb.2023.1146070. eCollection 2023.

Abstract

Microbial cell individuality is receiving increasing interest in the scientific community. Individual cells within clonal populations exhibit noticeable phenotypic heterogeneity. The advent of fluorescent protein technology and advances in single-cell analysis has revealed phenotypic cell variant in bacterial populations. This heterogeneity is evident in a wide range of phenotypes, for example, individual cells display variable degrees of gene expression and survival under selective conditions and stresses, and can exhibit differing propensities to host interactions. Last few years, numerous cell sorting approaches have been employed for resolving the properties of bacterial subpopulations. This review provides an overview of applications of cell sorting to analyze Salmonella lineage-specific traits, including bacterial evolution studies, gene expression analysis, response to diverse cellular stresses and characterization of diverse bacterial phenotypic variants.

Keywords: Salmonella; bacterial lineages; cell sorting; flow cytometry; phenotypic heterogeneity.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria*
  • Gene Expression Profiling
  • Phenotype
  • Salmonella* / genetics

Grants and funding

This work was supported by the grant US-1380232 from Programa Operativo FEDER 2014-2020 and Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía, the grants PID2020-116995RB-I00 and PID2019-104784RJ-I00 funded by MCIN/AEI/10.13039/5011100011033 and, as appropriate, by "ERDF A way of making Europe", by the "European Union" or by the "European Union Next Generation EU/PRTR" and the VI Plan Propio de Investigación y Transferencia from the Universidad de Sevilla.