Angiotensin-(1-7) mediated calcium signalling by MAS

Priyavathi Sureshkumar; Robson Augusto Souza Dos Santos; Natalia Alenina; Stefan Mergler; Michael Bader

doi:10.1016/j.peptides.2023.171010

Angiotensin-(1-7) mediated calcium signalling by MAS

Peptides. 2023 Jul:165:171010. doi: 10.1016/j.peptides.2023.171010. Epub 2023 Apr 13.

Authors

Priyavathi Sureshkumar¹, Robson Augusto Souza Dos Santos², Natalia Alenina³, Stefan Mergler⁴, Michael Bader⁵

Affiliations

¹ Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert-Rössle-Straße 10, 13125 Berlin, Germany; Department of Ophthalmology, Charité Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Berlin Institute of Health, Humboldt-Universität zu Berlin, Berlin, Germany.
² National Institute of Science and Technology in Nanobiopharmaceutics, Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
³ Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert-Rössle-Straße 10, 13125 Berlin, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, Germany.
⁴ Department of Ophthalmology, Charité Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Berlin Institute of Health, Humboldt-Universität zu Berlin, Berlin, Germany.
⁵ Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert-Rössle-Straße 10, 13125 Berlin, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, Germany; Charité Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Berlin Institute of Health, Humboldt-Universität zu Berlin, Berlin, Germany; Institute for Biology, University of Lübeck, Germany. Electronic address: mbader@mdc-berlin.de.

PMID: 37059396
DOI: 10.1016/j.peptides.2023.171010

Abstract

The G protein-coupled receptor, MAS, is the receptor of the endogenous ligand, Angiotensin (Ang)-(1-7). It is a promising drug target since the Ang-(1-7)/MAS axis is protective in the cardiovascular system. Therefore, a characterization of MAS signalling is important for developing novel therapeutics for cardiovascular diseases. In this paper, we show that Ang-(1-7) increases intracellular calcium in transiently MAS-transfected HEK293 cells. The calcium influx induced by the activation of MAS is dependent on plasma membrane Ca²⁺ channels, phospholipase C, and protein kinase C. Specifically, we could demonstrate that MAS employs non-selective, transient receptor potential channels (TRPs) for calcium entry.

Keywords: Angiotensin; Calcium; MAS; Trp channel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium
HEK293 Cells
Humans
Proto-Oncogene Mas*
Proto-Oncogene Proteins* / metabolism

Substances

Proto-Oncogene Proteins
Proto-Oncogene Mas
angiotensin I (1-7)
Calcium