miR-6216 regulates neural stem cell proliferation by targeting RAB6B

Wen Li; Ruijie Ji; Yujian Lin; Xiang Cheng; Zixin Tang; Hui He; Lei Zhang; Jianbing Qin; Meiling Tian; Guohua Jin; Xinhua Zhang

doi:10.1016/j.neures.2023.04.003

miR-6216 regulates neural stem cell proliferation by targeting RAB6B

Neurosci Res. 2023 Sep:194:15-23. doi: 10.1016/j.neures.2023.04.003. Epub 2023 Apr 13.

Authors

Wen Li¹, Ruijie Ji¹, Yujian Lin¹, Xiang Cheng¹, Zixin Tang¹, Hui He¹, Lei Zhang¹, Jianbing Qin¹, Meiling Tian¹, Guohua Jin¹, Xinhua Zhang²

Affiliations

¹ Department of Human Anatomy, Institute of Neurobiology, Nantong University, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China; Co-Innovation Center of Neuroregeneration, Nantong University, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China; Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China.
² Department of Human Anatomy, Institute of Neurobiology, Nantong University, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China; Co-Innovation Center of Neuroregeneration, Nantong University, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China; Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, No.19 Qixiu Road, No.3 Building of Qixiu Campus, Nantong 226001, Jiangsu, PR China. Electronic address: zhangxinhua@ntu.edu.cn.

PMID: 37059126
DOI: 10.1016/j.neures.2023.04.003

Abstract

Neural stem cells (NSCs) are a class of self-renewing, multipotent and undifferentiated progenitor cells that retain the capacity to both glial and neuronal lineages. MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in stem cell fate determination and self-renewal. Our previous RNA-seq data indicated that the expression of miR-6216 was decreased in denervated hippocampal exosomes compared with normal. However, whether miR-6216 participates in regulating NSC function remains to be elucidated. In this study, we demonstrated that miR-6216 negatively regulates RAB6B expression. Forced overexpression of miR-6216 inhibited NSC proliferation, and overexpression of RAB6B promoted NSC proliferation. These findings suggest that miR-6216 played an important role in regulating NSC proliferation via targeting RAB6B, and improve the understanding of the miRNA-mRNA regulatory network that affects NSC proliferation.

Keywords: Apoptosis; MiR-6216; Neural stem cells; Proliferation; RAB6B.

MeSH terms

Cell Differentiation / physiology
Cell Proliferation / physiology
MicroRNAs* / genetics
MicroRNAs* / metabolism
Neural Stem Cells* / metabolism
Neurons / metabolism

Substances

MicroRNAs