Multifunctional carbon dots for glutathione detection and Golgi imaging

Aikun Liu; Haojie Cai; Zhibing Xu; Jinlei Li; Xiaoyu Weng; Changrui Liao; Jun He; Liwei Liu; Yiping Wang; Junle Qu; Hao Li; Jun Song; Jiaqing Guo

doi:10.1016/j.talanta.2023.124520

Multifunctional carbon dots for glutathione detection and Golgi imaging

Talanta. 2023 Jul 1:259:124520. doi: 10.1016/j.talanta.2023.124520. Epub 2023 Apr 6.

Authors

Aikun Liu¹, Haojie Cai¹, Zhibing Xu¹, Jinlei Li¹, Xiaoyu Weng¹, Changrui Liao¹, Jun He¹, Liwei Liu¹, Yiping Wang¹, Junle Qu¹, Hao Li², Jun Song³, Jiaqing Guo⁴

Affiliations

¹ State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University); College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, PR China.
² State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University); College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, PR China. Electronic address: lihao000000@163.com.
³ State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University); College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, PR China. Electronic address: songjun@szu.edu.cn.
⁴ State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University); College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, PR China. Electronic address: 523986726@qq.com.

PMID: 37058943
DOI: 10.1016/j.talanta.2023.124520

Abstract

Glutathione (GSH) is present in almost every cell in the body and plays various integral roles in many biological processes. The Golgi apparatus is a eukaryotic organelle for the biosynthesis, intracellular distribution, and secretion of various macromolecules; however, the mechanism of GSH in the Golgi apparatus has not been fully elucidated. Here, specific and sensitive sulfur-nitrogen co-doped carbon dots (SNCDs) with orange-red fluorescence was synthesized for the detection of GSH in the Golgi apparatus. The SNCDs have a Stokes shift of 147 nm and excellent fluorescence stability, and they exhibited excellent selectivity and high sensitivity to GSH. The linear response of the SNCDs to GSH was in the range of 10-460 μM (LOD = 0.25 μΜ). More importantly, we used SNCDs with excellent optical properties and low cytotoxicity as probes, and successfully realized golgi imaging in HeLa cells and GSH detection at the same time.

Keywords: GSH detection; Golgi imaging; Orange-red fluorescence carbon dots.

MeSH terms

Carbon / toxicity
Fluorescent Dyes* / toxicity
Glutathione
Golgi Apparatus
HeLa Cells
Humans
Limit of Detection
Nitrogen
Quantum Dots* / toxicity

Substances

Fluorescent Dyes
Carbon
Glutathione
Nitrogen