The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus

Xidan Xu; Chongqing Wang; Qingwen Xiao; Xu Huang; Yue Zhou; Xiang Luo; Yuxin Zhang; Xiaowei Xu; Qinbo Qin; Shaojun Liu

doi:10.3389/fgene.2023.1135006

The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus

Front Genet. 2023 Mar 28:14:1135006. doi: 10.3389/fgene.2023.1135006. eCollection 2023.

Authors

Xidan Xu¹, Chongqing Wang¹, Qingwen Xiao¹, Xu Huang¹, Yue Zhou¹, Xiang Luo¹, Yuxin Zhang¹, Xiaowei Xu¹, Qinbo Qin¹, Shaojun Liu¹

Affiliation

¹ State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, China.

Abstract

Established autotetraploids often have a highly stable meiosis with high fertility compared with neo-autotetraploids. The autotetraploid Carassius auratus (4n = 200, RRRR) (4nRR), which stemmed from whole-genome duplication of Carassius auratus red var. (2n = 100, RR) (RCC), produces diploid gametes with an adopted diploid-like chromosome pairing in meiosis and maintains the formation of autotetraploid lineages. In this study, we focused on Dmc1, a meiosis-specific recombinase during the prophase of meiosis I, and elaborated on the genetic variation, alternative transcription, expression characterization, and epigenetic modification of Dmc1 in RCC and 4nRR. Two original Dmc1 from RCC were identified in 4nRR, and two duplicated Dmc1 differences in genetic composition were observed in 4nRR. Furthermore, we only noticed that one original and one duplicated Dmc1 were expressed in RCC and 4nRR, respectively. However, both possessed identical gene expression profiles, differential expression of sexual dimorphism, and hypomethylation levels. These results indicated that the specific expression of duplicated Dmc1 may be involve in the progression of meiosis of the diploid-like chromosome pairing in autotetraploid Carassius auratus. Herein, the findings significantly increase knowledge of meiosis of autopolyploid fish and provide meaningful insights into genetic breeding in polyploidy fish.

Keywords: DMC1; alternative transcription; autopolyploidization; genetic/epigenetic diversity; meiosis.

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant No. 32172972), postgraduate research and innovation project (5021800-202100002097), the Science and Technology Innovation Program of Hunan Province (Grant No. 2021RC4028), the National Natural Science Foundation of China (Grant No. U19A2040, 31730098), the Earmarked Fund for China Agriculture Research System (Grant No. CARS-45), the Hunan Provincial Science and Technology Department (2019RS5001), the Special Funds for Construction of Innovative Provinces in Hunan Province (2021NK1010).