Effect of end-inspiratory pause on airway and physiological dead space in anesthetized horses

Abstract

Objective: To evaluate the impact of a 30% end-inspiratory pause (EIP) on alveolar tidal volume (V_Talv), airway (V_Daw) and physiological (V_Dphys) dead spaces in mechanically ventilated horses using volumetric capnography, and to evaluate the effect of EIP on carbon dioxide (CO₂) elimination per breath (Vco₂br^-1), PaCO_2, and the ratio of PaO₂-to-fractional inspired oxygen (PaO₂:FiO₂).

Study design: Prospective research study.

Animals: A group of eight healthy research horses undergoing laparotomy.

Methods: Anesthetized horses were mechanically ventilated as follows: 6 breaths minute^-1, tidal volume (V_T) 13 mL kg^-1, inspiratory-to-expiratory time ratio 1:2, positive end-expiratory pressure 5 cmH₂O and EIP 0%. Vco₂br^-1 and expired tidal volume (V_TE) of 10 consecutive breaths were recorded 30 minutes after induction, after adding 30% EIP and upon EIP removal to construct volumetric capnograms. A stabilization period of 15 minutes was allowed between phases. Data were analyzed using a mixed-effect linear model. Significance was set at p < 0.05.

Results: The EIP decreased V_Daw from 6.6 (6.1-6.7) to 5.5 (5.3-6.1) mL kg^-1 (p < 0.001) and increased V_Talv from 7.7 ± 0.7 to 8.6 ± 0.6 mL kg^-1 (p = 0.002) without changing the V_TE. The V_Dphys to V_TE ratio decreased from 51.0% to 45.5% (p < 0.001) with EIP. The EIP also increased PaO₂:FiO₂ from 393.3 ± 160.7 to 450.5 ± 182.5 mmHg (52.5 ± 21.4 to 60.0 ± 24.3 kPa; p < 0.001) and Vco₂br^-1 from 0.49 (0.45-0.50) to 0.59 (0.45-0.61) mL kg^-1 (p = 0.008) without reducing PaCO₂.

Conclusions and clinical relevance: The EIP improved oxygenation and reduced V_Daw and V_Dphys, without reductions in PaCO₂. Future studies should evaluate the impact of different EIP in healthy and pathological equine populations under anesthesia.

Keywords: Bohr equation; airway dead space; end-inspiratory pause; physiological dead space; volumetric capnography.