What voltage-sensing phosphatases can reveal about the mechanisms of ion channel regulation by phosphoinositides

Yasushi Okamura; Daisuke Yoshioka

doi:10.1042/BST20221065

What voltage-sensing phosphatases can reveal about the mechanisms of ion channel regulation by phosphoinositides

Biochem Soc Trans. 2023 Apr 26;51(2):827-839. doi: 10.1042/BST20221065.

Authors

Yasushi Okamura¹, Daisuke Yoshioka¹

Affiliation

¹ Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Yamada Oka 2-2, Suita, Osaka 565-0871, Japan.

PMID: 37052219
DOI: 10.1042/BST20221065

Abstract

Many membrane proteins including ion channels and ion transporters are regulated by membrane phospholipids such as phosphoinositides in cell membranes and organelles. Voltage-sensing phosphatase, VSP, is a voltage-sensitive phosphoinositide phosphatase which dephosphorylates PI(4,5)P2 into PI(4)P. VSP rapidly reduces the level of PI(4,5)P2 upon membrane depolarization, thus serving as a useful tool to quantitatively study phosphoinositide-regulation of ion channels and ion transporters using a cellular electrophysiology system. In this review, we focus on the application of VSPs to Kv7 family potassium channels, which have been important research targets in biophysics, pharmacology and medicine.

Keywords: ion channel; membrane potential; phosphoinositide.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Cell Membrane / metabolism
Ion Channels / metabolism
Phosphatidylinositol 4,5-Diphosphate / metabolism
Phosphatidylinositols* / metabolism
Phosphoric Monoester Hydrolases* / metabolism

Substances

Phosphoric Monoester Hydrolases
Phosphatidylinositols
Phosphatidylinositol 4,5-Diphosphate
Ion Channels