Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome

Changjiang Fan; Xinyue Meng; Wei Yang; Peiyan Wang; Wenguang Chang; Peifeng Li; Jianxun Wang

doi:10.1039/d2cc06273a

Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome

Chem Commun (Camb). 2023 Apr 25;59(34):5106-5109. doi: 10.1039/d2cc06273a.

Authors

Changjiang Fan^{1

2}, Xinyue Meng^{1

2}, Wei Yang^{1

2}, Peiyan Wang^{1

2}, Wenguang Chang¹, Peifeng Li¹, Jianxun Wang^{1

2}

Affiliations

¹ Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China. cjfan@qdu.edu.cn.
² School of Basic Medicine, College of Medicine, Qingdao University, Qingdao 266071, Shandong, P. R. China. wangjx@qdu.edu.cn.

PMID: 37039083
DOI: 10.1039/d2cc06273a

Abstract

A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.

MeSH terms

Guanine
MicroRNAs*
Transcriptome

Substances

8-hydroxyguanine
MicroRNAs
2-acetylpyridine semicarbazone
Guanine