Genetic Variants of the Gsr Gene (rs2978663) and the Progression of Osteoporosis

Hadi Sajid Abdulabbas; Yasir Haider Al-Mawlah; Sahar Dakhil Yonis; Salah Hashim Shaheed

doi:10.5455/aim.2023.31.37-40

Genetic Variants of the Gsr Gene (rs2978663) and the Progression of Osteoporosis

Acta Inform Med. 2023 Mar;31(1):37-40. doi: 10.5455/aim.2023.31.37-40.

Authors

Hadi Sajid Abdulabbas¹, Yasir Haider Al-Mawlah², Sahar Dakhil Yonis³, Salah Hashim Shaheed⁴

Affiliations

¹ Continuous Education Department, Faculty of Dentistry, University of Al-Ameed, Karbala, Iraq.
² DNA Research Center, University of Babylon, Babylon, Iraq.
³ Department of Anatomy, College of Medicine, University of Al-Qadisiyah. Al-Qadisiyah, Iraq.
⁴ Department of Medical Laboratory Technique, Al-Safwa University College, Kerbala, Iraq.

Abstract

Background: Osteoporosis, demonstrated as an associated disease with more than 30 gene disorders, is a polygenic disorder, and it's also implicated in bone mineral density (BMD) regulations. Lipid peroxidation and hydrogen peroxide levels significantly increased and vice versa the antioxidant enzymes decreased such as Glutathione S-Reductase GSR was found in female postmenopausal females.

Objective: This research was done in order to find out the effect of rs2978663 genotypes on the progression of osteoporosis.

Methods: First, blood samples were used to extract DNA for analysis. Molecular examination was achieved using PCR, RFLP, and UV imaging after electrophoresis in an agarose gel, and these results were analyzed by SPSS (version 23).

Results: The genotypes differed in healthy people, and the proportions varied, as they were: the highest percentage was represented by the GA genotype (78%), followed by the AA genotype (16%), and the GG genotype (6%). For case samples, the highest percentage was represented by the GA genotype (51%), followed by the AA genotype (30%), and the GG genotype (19%). There are significant associations between GA genotype and restriction of fragility disease. The risk of having osteoporosis was significantly lower in those with the GA genotype (OR = 0.1946; 95% CI = 0.04-0.95; P = 0.03). The A allele frequency of the GSR gene (rs2978663) did not change significantly between study groups (OR: 0.9965, 95% CI: 0.5547-1.7816, P value: 0.9905).

Conclusion: Overall, it is safe to say that GSR-int3 (rs-2678663) was shown to have no association with osteoporosis in this research of Iraqi women. Inherent variation in the GSR gene (rs-2678663) is associated with decreased osteoporosis risk.

Keywords: ApaLI; GSR gene polymorphism; RFLP-PCR techniques; SNP; re2978663.