Prevalence and risk factors of Klebsiella spp. in milk samples from dairy cows with mastitis-A global systematic review

Jinming Song; Wentao Xiang; Qi Wang; Jiying Yin; Tian Tian; Qizhu Yang; Meng Zhang; Guiyang Ge; Jianming Li; Naichao Diao; Fei Liu; Kun Shi; Ruopeng Cai; Rui Du; Qinglong Gong

doi:10.3389/fvets.2023.1143257

Prevalence and risk factors of Klebsiella spp. in milk samples from dairy cows with mastitis-A global systematic review

Front Vet Sci. 2023 Mar 22:10:1143257. doi: 10.3389/fvets.2023.1143257. eCollection 2023.

Authors

Jinming Song¹, Wentao Xiang¹, Qi Wang¹, Jiying Yin¹, Tian Tian¹, Qizhu Yang¹, Meng Zhang¹, Guiyang Ge¹, Jianming Li², Naichao Diao², Fei Liu¹, Kun Shi², Ruopeng Cai¹, Rui Du^{1

2}, Qinglong Gong¹

Affiliations

¹ College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
² College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Abstract

Introduction: The overall prevalence of Klebsiella spp., a group of important zoonotic pathogens, in the global dairy herds and the risk of cross-species transmission between humans and dairy cows remain to be clarified. This systematic review aimed to determine the prevalence of Klebsiella spp. in milk samples from dairy cows with mastitis worldwide and to assess the factors influencing the prevalence of these strains.

Methods: Qualified studies published from 2007 to 2021 were retrieved from ScienceDirect, Web of Science, PubMed, WanFang Database, China National Knowledge Infrastructure (CNKI), and VIP Chinese Journal Database. Calculations of prevalence and their 95% confidence intervals (CIs) were performed for all the studies using the Freeman-Tukey double arcsine transformation (PFT).

Results: A total of 79,852 milk samples from 55 manuscripts were examined in this meta-analysis, and 2,478 samples were found to be positive for Klebsiella spp. The pooled prevalence estimates worldwide were 7.95% (95% CI: 6.07%-10.06%), with significant heterogeneity (I ² = 98.8%, p = 0). The sampling period of 2013-2020 had a higher (p < 0.05) Klebsiella-positive proportion of milk samples (12.16%, 95% CI: 8.08%-16.90%) than that of 2007-2012 (3.85%, 95% CI: 2.67%-5.21%), indicating that bovine mastitis caused by Klebsiella may become increasingly prevalent. The risk factors for the high prevalence of Klebsiella in milk samples mainly included: economic development level (developing countries; 11.76%, 95% CI: 8.25%-15.77%), mastitis type (CM; 11.99%, 95% CI: 8.62%-15.79%), and population density (>500 per sq km; 10.28%, 95% CI: 2.73%-21.58%). Additionally, a bivariate meta-regression analysis revealed that the multidrug-resistance (MDR) rate of the epidemic strains was also closely related to economic development level (R ² = 78.87%) and population density (R ² = 87.51%).

Discussion: Due to the potential risk of cross-species transmission between humans and cows, the prevalence of mastitis milk-derived Klebsiella and its high MDR rate need to be monitored, especially in developing countries with high population densities.

Keywords: Klebsiella; bovine mastitis; meta-analysis; multidrug-resistance (MDR); prevalence.

Publication types

Systematic Review

Grants and funding

This work was financially supported through grants from the National Natural Science Foundation of China (No. 32102677), the Science and Technology Project of the Jilin Provincial Education Department (No. JJKH20210364KJ), the Natural Science Foundation of Jilin Province (No. YDZJ202201ZYTS449), and the China Postdoctoral Science Foundation (No. 2021M691224).