Impact of Mesoporous Silica Functionalization Fine-Tuning on Antibiotic Uptake/Delivery and Bactericidal Activity

Karim Bouchmella; Quentin Lion; Christel Gervais; Mateus Borba Cardoso

doi:10.1021/acsomega.2c08065

Impact of Mesoporous Silica Functionalization Fine-Tuning on Antibiotic Uptake/Delivery and Bactericidal Activity

ACS Omega. 2023 Mar 22;8(13):12154-12164. doi: 10.1021/acsomega.2c08065. eCollection 2023 Apr 4.

Authors

Karim Bouchmella^{1

2

3}, Quentin Lion^{1

4

5

6}, Christel Gervais⁷, Mateus Borba Cardoso^{1

2}

Affiliations

¹ Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), P.O. Box 6192, Campinas CEP 13083-970, SP, Brazil.
² Chemistry Institute (IQ), Universidade Estadual de Campinas (UNICAMP), P.O. Box 6154, Campinas CEP 13083-970, SP, Brazil.
³ ICGM, University Montpellier, CNRS, ENSCM, 34095 Montpellier, France.
⁴ Interdisciplinary Cluster for Applied Genoproteomics, University of Liege, Liege 4000, Belgium.
⁵ Laboratory of Medical Chemistry, University of Liege, Liege 4000, Belgium.
⁶ GIGA Stem Cells, University of Liege, Liege 4000, Belgium.
⁷ LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris, Sorbonne Université, 4 place Jussieu, 75252 Paris Cedex 05, France.

Abstract

The mesoporous SBA-15 material was surface-functionalized with amino and carboxylic acid groups and used as a platform to investigate the interaction of these chemical groups with tetracycline, kanamycin, and ampicillin antibiotics. The interactions between the antibiotic and the functionalized surfaces were characterized using two-dimensional ¹H-¹³C HETCOR CP MAS and FTIR spectroscopy and indicated that -COO^- NH₃ ⁺ bondings had been formed between chemical groups on the silica surface and drug molecules. The surface modification resulted in higher kanamycin and ampicillin loadings and a slow-release rate, and all synthesized systems showed antibacterial activity against susceptible Escherichia coli bacteria. Almost total death of bacteria was obtained using a few ppm of tetracycline- and kanamycin-loaded systems, whereas the ampicillin-loaded one showed lower bactericidal activity than free ampicillin.