Combination of PDGF-BB and adipose-derived stem cells accelerated wound healing through modulating PTEN/AKT pathway

Fangyuan Lai; Shijie Dai; Ye Zhao; Yi Sun

doi:10.1016/j.injury.2023.02.027

Combination of PDGF-BB and adipose-derived stem cells accelerated wound healing through modulating PTEN/AKT pathway

Injury. 2023 Feb 13:S0020-1383(23)00123-7. doi: 10.1016/j.injury.2023.02.027. Online ahead of print.

Authors

Fangyuan Lai¹, Shijie Dai², Ye Zhao¹, Yi Sun³

Affiliations

¹ Center for Plastic & Reconstructive Surgery, Department of Plastic & Reconstructive Surgery, Zhejiang Provincial People's Hospital （Affiliated People's Hospital, Hangzhou Medical College）, Hangzhou, Zhejiang, China.
² College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
³ Center for Plastic & Reconstructive Surgery, Department of Plastic & Reconstructive Surgery, Zhejiang Provincial People's Hospital （Affiliated People's Hospital, Hangzhou Medical College）, Hangzhou, Zhejiang, China. Electronic address: sunyi@hmc.edu.cn.

PMID: 37028952
DOI: 10.1016/j.injury.2023.02.027

Abstract

Adipose-derived stem cells (ADSCs) have been widely proven to facilitate wound healing. Our study aimed to estimate the influence of combined ADSCs and platelet-derived growth factor-BB (PDGF-BB) on wound healing. We utilized 4 healthy SD rats to isolate ADSCs. Platelet-rich plasma (PRP) was acquired utilizing a two-step centrifugation technology. The role of PRP, PDGF-BB, and PDGF-BB combined with a PI3k inhibitor LY294002 on the viability, migration, and PTEN/AKT pathway in ADSCs were examined utilizing CCK-8, Transwell, and western blot assays. Then, we constructed an open trauma model in SD rats. Effects of ADSCs treated with PDGF-BB on pathological changes, CD31, and PTEN/AKT pathway of wound closure were assessed by hematoxylin & eosin (H&E) staining, Masson staining, immunohistochemical, and western blot assays, respectively. PRP and PDGF-BB intensified the viability and migration of ADSCs by modulating the PTEN/AKT pathway. Interestingly, LY294002 reversed the role of PDGF-BB on ADSCs. In vivo experiments, combined intervention with ADSCs plus PDGF-BB/PRP facilitated wound closure and ameliorated histological injury. Moreover, combined intervention with ADSCs and PDGF-BB attenuated the PTEN level and elevated the CD31 level as well as the ratio of p-AKT/AKT in the skin tissues. A combination of ADSCs and PDGF-BB facilitated wound healing might associate with the regulation of the PTEN/AKT pathway.

Keywords: Adipose-derived stem cells; PDGF-BB; PRP; Wound healing.