Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment

Anne-Laure Boutigny; Benoit Remenant; Bruno Legendre; Véronique Beven; Mathieu Rolland; Yannick Blanchard; Amandine Cunty

doi:10.1016/j.mimet.2023.106719

Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment

J Microbiol Methods. 2023 May:208:106719. doi: 10.1016/j.mimet.2023.106719. Epub 2023 Apr 5.

Authors

Anne-Laure Boutigny¹, Benoit Remenant², Bruno Legendre², Véronique Beven³, Mathieu Rolland², Yannick Blanchard³, Amandine Cunty²

Affiliations

¹ Anses, Plant Health Laboratory, Bacteriology Virology GMO Unit, 7 rue Jean Dixméras, 49044 Angers cedex 01, France. Electronic address: anne-laure.boutigny@anses.fr.
² Anses, Plant Health Laboratory, Bacteriology Virology GMO Unit, 7 rue Jean Dixméras, 49044 Angers cedex 01, France.
³ Anses, Ploufragan Laboratory, Viral Genetics and Biosafety Unit, BP 53, 22440 Ploufragan, France.

PMID: 37028518
DOI: 10.1016/j.mimet.2023.106719

Abstract

A targeted enrichment method was developed to sequence Xylella fastidiosa genomic DNA directly from plant samples. The method was evaluated on various plant species infected with different strains at different levels of contamination. After enrichment, X. fastidiosa genome coverage was above 99.9% for all tested samples.

Keywords: Capture; Illumina sequencing; SureSelect; Whole genome; Xylella fastidiosa.

MeSH terms

Plant Diseases*
Plants
Sequence Analysis, DNA
Whole Genome Sequencing
Xylella* / genetics

Supplementary concepts

Xylella fastidiosa