Generation of an induced pluripotent stem cell lines NSHDMUi001-A from patients with type 2 diabetes

Peixue Li; Chun Wang; Jiatian Wang; Jing Tan; Yuzhu Liu; Yongzhong Lin

doi:10.1016/j.scr.2023.103087

Generation of an induced pluripotent stem cell lines NSHDMUi001-A from patients with type 2 diabetes

Stem Cell Res. 2023 Jun:69:103087. doi: 10.1016/j.scr.2023.103087. Epub 2023 Mar 28.

Authors

Peixue Li¹, Chun Wang¹, Jiatian Wang¹, Jing Tan¹, Yuzhu Liu¹, Yongzhong Lin²

Affiliations

¹ The Second Hospital of Dalian Medical University, China.
² The Second Hospital of Dalian Medical University, China. Electronic address: lin19671024@163.com.

PMID: 37028179
DOI: 10.1016/j.scr.2023.103087

Abstract

Type 2 diabetes mellitus (T2DM) is common in China, and its aetiology and pathogenesis are still unclear. We reprogrammed pEP4EO2SEN2K and pEP4EO2SET2K, pCEP4-M2L was electrotransfected in T2DM patients with pEP4EO2SEN2K, and pCEP4-M2L was electrotransfected in T2DM patients expressing the OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT transcription factors to obtain induced pluripotent stem cells (iPSCs). The obtained iPSCs have been verified to have pluripotency, normal karyotype and differentiation potential; therefore, these cells can be used in the study of disease pathophysiology and drug development to create new therapeutic targets for T2DM and associated central nervous system damage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Diabetes Mellitus, Type 2* / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Kruppel-Like Factor 4

Substances

Kruppel-Like Factor 4